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Updated: Jun 11, 2026

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Published on: February 11, 2019

Deterministic cell pairing with simultaneous microfluidic merging and sorting of droplets.

Kevin M Joslin¹, Sophia Dateshidze¹, Seung Won Shin¹

¹Department of Bioengineering, California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, California, USA. iain.c.clark@berkeley.edu.

|October 1, 2025

Summary

Researchers developed a microfluidic platform, Pair Isolation by Coalescence and Sorting (PICS), to precisely create cell pairs for studying cell crosstalk. This method significantly improves the isolation of desired cell combinations for biological research.

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Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Cell-cell interactions are crucial for biological processes like immune response and tissue repair.
Existing methods struggle to generate specific cell pairs in high throughput for studying cell crosstalk.
Random loading in droplet microfluidics yields low percentages of desired cell combinations.

Purpose of the Study:

To introduce a novel microfluidic platform, Pair Isolation by Coalescence and Sorting (PICS), for generating pre-defined cell pairs.
To enable high-throughput analysis of cell crosstalk by improving the isolation of specific cell combinations.
To demonstrate the utility of PICS for co-culture studies and downstream applications.

Main Methods:

PICS utilizes droplet microfluidics with fluorescence detection to identify and merge specific cell pairs.
Simultaneous electrocoalescence and dielectrophoretic sorting are employed to isolate desired merged droplets.
Cells are encapsulated in alginate hydrogels to maintain viability during co-culture studies.

Main Results:

PICS achieved 98.6% purity for merged and sorted fluorescent dye-loaded droplets.
>90% of desired cell pairs were recovered, a significant improvement over random loading (<1%).
Alginate hydrogels containing target cell pairs showed 93.3% purity and maintained cell viability for 18 hours.

Conclusions:

PICS is a flexible and efficient platform for enriching specific combinations of droplets, cells, or particles.
The platform enables cleaner downstream workflows by physically isolating desired droplets.
PICS offers advanced capabilities for high-throughput studies of cell crosstalk and multi-step assays.