Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.1K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.1K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.6K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural insights into target detection by the <i>S. marcescens</i> type III CRISPR complex and its deployment in SNP identification.

bioRxiv : the preprint server for biology·2026
Same author

Top-Down Mass Spectrometry-Based Structural and Functional Proteomics: A Perspective.

Journal of mass spectrometry : JMS·2026
Same author

Intact Proteoform Analysis by Capillary Electrophoresis-Mass Spectrometry. Are We There Yet?

Angewandte Chemie (International ed. in English)·2025
Same author

Methionine Oxidation Footprinting in Intact Proteins (MOFIP) Using Top-Down Proteomics.

Proteomics·2025
Same author

Characterization of the Intact Proteomic Profile of Senescent-Associated Secretory Phenotype by Top-Down Mass Spectrometry.

Analytical chemistry·2025
Same author

Top-Down Thermal Proteome Profiling (TD-TPP) for Functional Characterization of the Intact Proteoforms in Complex Samples.

Journal of mass spectrometry : JMS·2025
Same journal

Biodegradable Self-Powered Electrotherapy Patch for Integrated Smart Wound Management.

Analytical chemistry·2026
Same journal

Metabolite Fraction Libraries for Quantitative NMR Metabolomics.

Analytical chemistry·2026
Same journal

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.

Analytical chemistry·2026
Same journal

Overcoming the Debye Shielding Effect with Concave-Convex Structures for Sensitivity-Enhanced Thin-Film Transistors.

Analytical chemistry·2026
Same journal

Mode-Phase-Difference Photothermal Spectroscopy Assisted by a Bent Biconically Tapered Microfiber for Gas Sensing.

Analytical chemistry·2026
Same journal

Negative-Pressure-Actuated Microfluidics: A Dual-Mode Point-of-Care Sensor for Allergen-Specific IgE in Interstitial Fluid.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

10.0K

High-Throughput Top-Down Proteomic Analysis of Picogram-Level Complex Samples Using Multisegment Spray-Capillary

Zhitao Zhao1, Yanting Guo1, Kellye A Cupp-Sutton2

  • 1Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.

Analytical Chemistry
|October 1, 2025
PubMed
Summary
This summary is machine-generated.

This study enhances top-down proteomics by integrating multisegment sample injection with spray-capillary capillary electrophoresis-mass spectrometry (CE-MS). This boosts throughput for analyzing mass-limited samples like single cells.

More Related Videos

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

10.2K
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

7.7K

Related Experiment Videos

Last Updated: Jan 16, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

10.0K
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

10.2K
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

7.7K

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Top-down proteomics is crucial for PTM-regulated cellular functions in mass-limited samples.
  • Capillary electrophoresis (CE) offers high resolution, sensitivity, and speed for proteomics compared to LC.
  • Previous work introduced the Spray-Capillary for ultralow-volume CE-MS analysis.

Purpose of the Study:

  • To enhance throughput for mass-limited top-down proteomics.
  • To integrate multisegment sample injection with the spray-capillary CE-MS platform.
  • To enable high-throughput quantitative analysis of proteoforms.

Main Methods:

  • Developed multisegment sample injection for spray-capillary CE-MS.
  • Optimized spacer between sample plugs for baseline separation.
  • Utilized ESI-assisted device for quantitative ultralow-volume sampling.
  • Performed quantitative analysis of *E. coli* lysate.

Main Results:

  • Achieved baseline separation of multiple protein samples in a single CE-MS run.
  • Quantified *E. coli* lysate (10-250 pg) with strong linearity (R² > 0.98).
  • Supported up to 17 sample segments per run (approx. 90 min).

Conclusions:

  • The optimized platform significantly enhances throughput for mass-limited top-down proteomics.
  • Enables analysis of hundreds of mass-limited samples (e.g., single cells) daily.
  • Represents a substantial advancement for quantitative proteomic analyses.