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Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
Published on: December 15, 2017
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Developing a Pyridine-Imidazole-Modified Fluorescent Probe for Monitoring Carboxylesterase Level During the
Lu Tang1, Ruojun Man2, Weiling Deng1
1ENT Department, Jinhua Wucheng District People's Hospital, Jinhua, China.
Summary
A new fluorescent probe, PIM-CarE, effectively monitors carboxylesterase (CarE) levels in inflammation. This tool offers high sensitivity and selectivity for inflammation regulation studies.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Chemical Biology
Background:
- Carboxylesterase (CarE) plays a crucial role in biological processes.
- Monitoring CarE levels is important for understanding inflammation.
- Existing methods for CarE detection may have limitations.
Purpose of the Study:
- To develop a novel fluorescent probe for sensitive and selective detection of CarE.
- To investigate the probe's performance in monitoring inflammation in macrophages.
Main Methods:
- Synthesis of a pyridine-imidazole-modified fluorescent probe (PIM-CarE).
- Characterization of the probe's photophysical properties.
- Evaluation of probe's response to varying CarE levels.
- Testing probe's stability under different conditions (pH, time, temperature).
- Application of the probe in monitoring inflammation in macrophages.
Main Results:
- The ortho-pattern modification led to enhanced fluorescence intensity.
- PIM-CarE showed a dose-dependent fluorescence enhancement with increasing CarE levels.
- The probe exhibited a practical linear range, high sensitivity, and stable optical performance.
- High selectivity and low cytotoxicity were observed.
- Successful monitoring of inflammation generation and treatment in macrophages was achieved.
Conclusions:
- PIM-CarE is a promising fluorescent probe for monitoring CarE levels.
- The probe facilitates investigations into inflammation regulation.
- This tool has potential applications in various research departments studying inflammation.
