Search

Search

Home
...
Research Domains Biomedical and Clinical Sciences Oncology and Carcinogenesis Predictive and prognostic markers
Research Domains
Biomedical and Clinical Sciences
Oncology and Carcinogenesis
Predictive and prognostic markers
Pharmacological targeting of the IL-17/neutrophil axis attenuates calcific deposits in rat models of calciphylaxis

Pharmacological targeting of the IL-17/neutrophil axis attenuates calcific deposits in rat models of calciphylaxis

Bo Tao ^1,2,3,4,5,6, Edward Z Cao ^1,2,3,4,5,6, James Hyun ^1,2,3,4,5,6, Sivakumar Ramadoss ^1,2,3,4,5,6, Juan F Alvarez ^1,2,3,4,5,6, Lianjiu Su ^1,2,3,4,5,6, Qihao Sun ^1,2,3,4,5,6, Zhihao Liu ^1,2,3,4,5,6, Linlin Zhang ^1,2,3,4,5,6, Alejandro Espinoza ⁷, Yiqian Gu ^3,4,5,6, Feiyang Ma ⁸, Shen Li ^1,2,3,4,5,6, Matteo Pellegrini ^3,4,5,6, Arjun Deb ^1,2,3,4,5,6

Bo Tao ^1,2,3,4,5,6, Edward Z Cao ^1,2,3,4,5,6, James Hyun ^1,2,3,4,5,6

¹Division of Cardiology, Department of Medicine, David Geffen School of Medicine.
²UCLA Cardiovascular Theme, David Geffen School of Medicine.
³Department of Molecular, Cell and Developmental Biology, College of Letters and Sciences.
⁴Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research.
⁵Molecular Biology Institute.
⁶California Nanosystems Institute, and.
⁷Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, California, USA.
⁸Department of Cell and Developmental Biology, Northwestern University, Feinberg School of Medicine, Chicago, Illinois, USA.

⁰Division of Cardiology, Department of Medicine, David Geffen School of Medicine.

The Journal of Clinical Investigation +

|

October 1, 2025

|

October 1, 2025

Related Experiment Videos

Contact us if these videos are not relevant.

Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists

Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists

Published on: October 18, 2024

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model

A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model

Published on: November 18, 2022

See all related videos

View abstract on PubMed

Summary

This summary is machine-generated.

Calciphylaxis involves skin calcification driven by inflammation and immune cells. Targeting interleukin-17 (IL-17) and neutrophils shows promise in reducing calcific deposits in animal models.

Area Of Science

Dermatology
Immunology
Pathobiology

Background

Calciphylaxis is a rare, severe condition with poor survival and unclear mechanisms.
Ectopic calcification in skin and blood vessels characterizes this disorder.

Purpose Of The Study

To investigate the pathobiology of calciphylaxis using animal models.
To explore the role of inflammation and the immune system in disease development.

Main Methods

Developed animal models mimicking human calciphylaxis.
Assessed the impact of immune cell activation, immunosuppressants, and targeted therapies.
Analyzed cytokine and enzyme expression in affected tissues.

Main Results

Cutaneous calcification was preceded by inflammatory cell infiltration.
Immune cell-deficient rodents were resistant to calcification.
IL-17 and neutrophils were identified as key mediators.
Targeting IL-17 or neutrophils reduced calcific deposits.

Conclusions

Local inflammation, hypercalcemia, and hyperphosphatemia contribute to calciphylaxis.
The immune system, particularly the IL-17/neutrophil axis, plays a critical role.
Therapeutic strategies targeting these pathways may be beneficial.

Keywords:

Bone disease Cell biology Dermatology Neutrophils Skin

Related Experiment Videos

Contact us if these videos are not relevant.

Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists

Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists

Published on: October 18, 2024

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model

A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model

Published on: November 18, 2022

See all related videos

Related Concept Videos

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Share

X
Facebook
LinkedIn
YouTube

ABOUT JoVE

Overview
Leadership
Blog
JoVE Help Center

AUTHORS

Publishing Process
Editorial Board
Scope & Policies
Peer Review
FAQ
Submit

LIBRARIANS

Testimonials
Subscriptions
Access
Resources
Library Advisory Board
FAQ

RESEARCH

JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive

EDUCATION

JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Faculty Site

Terms & Conditions of Use
Privacy Policy
Policies