Related Experiment Video
Updated: Jan 6, 2026
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The complete genome assembly of Astragalus membranaceus: enabling more accurate genetic research
Huibin Qin1, Aohui Li2, Shuyu Zhong2
1Center for Agricultural Genetic Resources Research, Shanxi Agricultural University/Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences, Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan 030031, China.
Background:
Astragalus membranaceus (Fisch.) Bunge is a globally significant medicinal plant renowned for its potent immunomodulatory and antioxidant properties. However, the existing reference genome for this species remains incomplete, characterized by fragmented assemblies and the absence of centromeric and telomeric regions, thereby limiting comprehensive exploration of the genetic mechanisms underlying its key traits.
Findings:
We hereby present the first complete genome assembly for A. membranaceus (Fisch.) Bge "AM-T2T," achieved through the integration of PacBio HiFi, ultra-long Oxford Nanopore Technologies, and Hi-C sequencing. The assembly achieved a total size of 1.39 Gb with an N50 of 180.45 Mb. The genome exhibits remarkable completeness (99.63% BUSCO completeness; long terminal repeat assembly index of 22.67) and high accuracy (quality value of 57.51; Genome Continuity Inspector score of 36.23). It features annotations of 64.22% repetitive sequences, 16 telomeres, 8 centromeres, 32,600 high-confident genes, 248 cytochrome P450 monooxygenases (CYP450s), and 163 uridine diphosphate glycosyltransferases. Notably, 158.58 Mb of previously unassembled regions were resolved, harboring 4 CYP450s. Additionally, 2,267 unique genes and 20,652 conserved genes were identified within the AM-T2T genome. Comparative analysis with Astragalus mongholicus assembly revealed 1,413 structural variations.
Conclusions:
This complete genome assembly of A. membranaceus represents a significant advancement in the genomic characterization of A. membranaceus, providing a robust resource that will bolster genetic research, breeding programs, and medicinal applications.
More Related Videos
12:08Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
10:40Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
