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Oroxylin A exerts antiproliferative effects through downregulation of E6 and E7 oncogenes in cervical cancer HeLa

Shan Hu1, Yongting Lu2, Pratibha Pandey3

  • 1Department of Anesthesiology, The Taiyun Hospital of Peking University First Hospital/Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, 030000, China.

Naunyn-Schmiedeberg'S Archives of Pharmacology
|October 1, 2025
PubMed

Insights

Oroxylin A (OrA), a plant compound, effectively inhibits cervical cancer cell growth by targeting human papillomavirus (HPV) E6/E7 oncogenes. This leads to tumor suppressor gene activation and apoptosis, offering a promising natural therapeutic approach.

Area of Science:

  • Phytochemistry
  • Oncology
  • Molecular Biology

Background:

  • Cervical cancer progression is driven by HPV E6 and E7 oncogenes.
  • Plant-derived compounds, particularly flavonoids, show potential as natural anticancer agents.
  • Targeting HPV oncogenes is a key therapeutic strategy for cervical cancer.

Purpose of the Study:

  • To investigate the anti-cervical cancer effects of oroxylin A (OrA).
  • To elucidate the molecular mechanisms of OrA action, including its impact on HPV E6/E7, apoptosis, and tumor suppressor genes.

Main Methods:

  • MTT assay for cell proliferation
  • Cell cycle arrest analysis
  • Hoechst staining for apoptosis
  • Molecular docking studies
  • RT-PCR for gene expression analysis (E6/E7, p53, pRb, Bcl-2, Bax, caspases)

Main Results:

  • Oroxylin A significantly inhibited HeLa cell proliferation in a dose-dependent manner.
  • OrA downregulated HPV E6/E7 oncogene expression.
  • OrA upregulated tumor suppressor proteins p53 and pRb.
  • OrA induced apoptosis through p53-mediated pathways, activating proapoptotic genes and caspases.

Conclusions:

  • Oroxylin A demonstrates significant anticancer activity against cervical cancer by targeting HPV E6/E7 oncogenes.
  • OrA promotes apoptosis and inhibits proliferation via the p53/pRb tumor suppressor pathway.
  • OrA represents a promising natural compound for cervical cancer therapy.
Keywords:
AnticancerApoptosisCervical cancerNatural productOroxylin A

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