Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Therapy00:59

Gene Therapy

27.3K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
27.3K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Experimental RNAi02:15

Experimental RNAi

7.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.3K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

8.6K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.6K
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

982
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
982
Improving Translational Accuracy02:07

Improving Translational Accuracy

14.1K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
14.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uncovering Hidden Prognostic Patterns in Colorectal Cancer Histology Using Unsupervised Learning: A Computational Pathology Study.

Bioengineering (Basel, Switzerland)·2026
Same author

Impact of the COVID-19 Pandemic on Routine Childhood Vaccination in Oklahoma.

Vaccines·2026
Same author

Correction to "Active Targeting Significantly Outperforms Nanoparticle Size in Facilitating Tumor-Specific Uptake in Orthotopic Pancreatic Cancer".

ACS applied materials & interfaces·2026
Same author

Evaluation of Ferroptosis as a Biomarker to Predict Treatment Outcomes of Cancer Immunotherapy.

Cancer research communications·2025
Same author

Cutting edge: Extrinsic and intrinsic sex effects differentially regulate pulmonary ILC2 numbers, phenotype, and function.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same author

Interferon-β treatment reverses the detrimental effect of B-cell depletion therapy on respiratory virus infection.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same journal

Erratum for the Research Article "Assessing the health risks of rice cadmium content standards in China" by H. Chu <i>et al</i>.

Science advances·2026
Same journal

Erratum for the Research Article "Developmental regulation of Erk signaling by mitotic kinases" by F. Chen <i>et al</i>.

Science advances·2026
Same journal

Magnetically levitated metasurface enabling tangible and bidirectional human-machine interaction.

Science advances·2026
Same journal

A general photoinduced manganese-catalyzed platform for the sequential difunctionalization of [1.1.1]propellane.

Science advances·2026
Same journal

Turning sound and force into light with AlN:Mn<sup>2+</sup> mechanoluminescence.

Science advances·2026
Same journal

Extreme dominance of Earth-origin heavy ions in the intense ring current near the Earth during the May 2024 super geomagnetic storm.

Science advances·2026
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy
09:56

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Published on: February 21, 2025

1.3K

Enhancing mRNA therapy through iterative delivery.

Joshua Seaberg1, Suresh Kumar Gulla2, Jocelyn Labombarde1

  • 1Department of Pathology, University of Oklahoma Health Sciences, Oklahoma City, OK 73104, USA.

Science Advances
|October 1, 2025
PubMed
Summary
This summary is machine-generated.

A novel delivery system using gold nanoparticles and messenger RNA (mRNA) enhances tumor targeting and therapeutic effects. This "Aurniosomes" system improves protein expression and efficacy in solid tumors.

More Related Videos

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

10.6K
In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells
10:07

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells

Published on: November 13, 2014

25.6K

Related Experiment Videos

Last Updated: Jan 16, 2026

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy
09:56

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Published on: February 21, 2025

1.3K
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

10.6K
In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells
10:07

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells

Published on: November 13, 2014

25.6K

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Oncology

Background:

  • Clinical translation of therapeutic messenger RNA (mRNA) for solid tumors is hindered by inadequate delivery systems.
  • Effective delivery is crucial for enhancing mRNA's therapeutic potential in cancer treatment.

Purpose of the Study:

  • To develop and evaluate a novel mRNA delivery system for improved efficacy in solid tumors.
  • To investigate the mechanisms underlying the enhanced delivery and therapeutic effects of the novel system.

Main Methods:

  • Co-encapsulation of gold nanoparticles (AuNPs) and mRNA within non-ionic surfactant vehicles (NSVs) to create "Aurniosomes" (AuNSVs).
  • In vitro and in vivo studies to assess vector accumulation, cellular uptake, protein expression, and therapeutic efficacy.
  • Mechanistic studies involving clathrin-mediated endocytosis (CME), caveolin-mediated endocytosis (CvME), and endolysosomal trafficking pathways.

Main Results:

  • AuNSVs demonstrated improved accumulation and uptake within tumors compared to conventional systems.
  • Enhanced protein expression and significant therapeutic efficacy were observed in both in vitro and in vivo models.
  • Mechanistic studies revealed an iterative delivery process involving AuNP-mediated inactivation of cellular regulators, promoting further AuNSV entry and endosomal escape.

Conclusions:

  • AuNSVs represent a promising next-generation mRNA delivery platform for solid tumors.
  • The unique iterative delivery mechanism enhances mRNA bioavailability and therapeutic outcomes.
  • This approach holds potential for advancing mRNA-based cancer therapies.