Related Experiment Video
Updated: Jan 16, 2026
Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
Published on: February 11, 2021
A Single Bout of High-Intensity Running Exercise Transiently and Reversibly Increases Intestinal Permeability and
Saki Kondo1,2, Takahiro Nagatake1,3, Koji Hosomi1,4
1Laboratory of Vaccine Materials and Laboratory of Gut Environmental System, Microbial Research Center for Health and Medicine, National Institutes of Biomedical Innovation, Health and Nutrition (NIBN), Ibaraki, Osaka, JAPAN.
Introduction:
Although the health benefits of exercise are well-documented, intense exercise can induce gastrointestinal symptoms, potentially due to disruption of the intestinal barrier. This disruption allows foreign substances to enter the intestine and circulation, triggering immune system activation and inflammation. In this study, we investigated the effects of a single bout of running exercise at different intensities on intestinal permeability and systemic immune cell responses.
Methods:
Male C57BL/6J mice were assigned to a control group, a low-intensity exercise group (10 m/min, Ex-low), and a high-intensity exercise group (20 m/min, Ex-high). The exercise lasted 60 min, while the control group remained sedentary. Immediately after exercise, intestinal permeability was assessed using FITC-Dextran. Immune cells were isolated from the lungs, blood, small intestinal lamina propria (siLP), and large intestinal lamina propria (liLP), while neutrophils, macrophages, eosinophils, B cells, and T cells were quantified by flow cytometry.
Results:
The Ex-high group exhibited significantly increased intestinal permeability and neutrophil infiltration in the siLP compared with both the control and Ex-low groups. No significant differences in other immune cell types were observed between the groups. Furthermore, the Ex-high group demonstrated elevated neutrophil counts in the liLP, lungs, and blood. The expression of inflammatory cytokines ( Il-1β and Il-6 ) and chemokines ( Cxcl1 and Cxcl2 ) was also significantly higher in the small intestines of the Ex-high group.
Conclusions:
A single bout of high-intensity exercise increases intestinal permeability and neutrophil infiltration in both the intestines and systemic tissues, highlighting a potential mechanism by which intense exercise induces intestinal and systemic inflammation.
