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Gene editing techniques in cancer research

Nurhasni Hasan1, Juliana Palungan1, Muneeb Ullah2

  • 1Faculty of Pharmacy, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km 10, Makassar, Indonesia.

Methods in Cell Biology
|October 1, 2025
PubMed

Insights

Gene editing technologies like CRISPR-Cas9 offer new ways to understand tumors and develop personalized cancer therapies. This research explores methods, challenges, and advancements in applying gene editing to target cancer-causing genes.

Area of Science:

  • Oncology
  • Genetics
  • Biotechnology

Background:

  • Gene editing is revolutionizing cancer research by offering insights into tumor biology.
  • It holds promise for developing targeted cancer medicines and therapies.
  • Understanding different gene editing techniques is crucial for advancing cancer treatment.

Purpose of the Study:

  • To review and compare major gene editing techniques: Zinc Finger Nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), and CRISPR-Cas9.
  • To analyze protocol modifications, utility, efficiency, and application challenges of these methods in high-prevalence tumors.
  • To discuss current advancements in targeting oncogenes and tumor suppressor genes for precision cancer therapy.

Main Methods:

  • Comparative analysis of ZFNs, TALENs, and CRISPR-Cas9 systems.
  • Review of protocol modifications specific to different tumor types.
  • Examination of efficiency, utility, and application challenges in oncology.

Main Results:

  • Detailed discussion on the specific protocols and modifications for each gene editing technique.
  • Evaluation of the effectiveness and limitations of ZFNs, TALENs, and CRISPR-Cas9 in targeting cancer genes.
  • Identification of current progress in refining gene editing tools for oncogene and tumor suppressor gene targeting.

Conclusions:

  • Gene editing techniques are pivotal in advancing precision cancer therapy.
  • Further development is needed to overcome challenges and enhance the application of these tools in oncology.
  • These technologies are key to unlocking new therapeutic strategies for various cancers.
Keywords:
CRISPR-Cas9Gene editingTALENsZFN

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