A Superb Antifouling Electrochemical Biosensor Based on Macrocycle Encapsulation Strategy for Biomarker Analysis in...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Materials Science

Background:

• Electrode biofouling and enzymatic degradation hinder biomarker analysis in biological fluids.
• Developing robust biosensors for complex environments remains a challenge.

Purpose of the Study:

• To create a stable and antifouling sensing interface for precise biomarker detection.
• To enhance peptide stability and resistance to proteolytic degradation.

Main Methods:

• Host-guest interaction strategy using quaterphen[4]arene sulfate (WQP[4]S) to encapsulate a peptide recognition unit.
• Development of a ratiometric electrochemical biosensor incorporating methylene blue (MB) as a reference.
• Testing the biosensor's performance in blood samples.

Main Results:

• The WQP[4]S-peptide complex showed excellent antifouling and proteolytic resistance.
• The ratiometric electrochemical biosensor achieved high selectivity and sensitivity for Furin detection.
• A low detection limit of 0.81 U L⁻¹ was obtained, with accurate assays in human blood samples.

Conclusions:

• The developed host-guest complex strategy provides a robust approach for antifouling and enzymatic-resistant biosensors.
• This method enables precise biomarker detection in complex biological fluids.
• The biosensor shows promise for clinical applications, including diabetes patient monitoring.