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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jan 16, 2026

Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization

Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization

Published on: April 7, 2023

Identifying ovarian cortex cell subpopulations using multicolor flow cytometry.

Sophie Frontczak^1,2,3, Tristan Zver^4,5, Jean-Baptiste Pretalli⁶

¹Université Marie et Louis Pasteur, CHU Besançon, Besançon, F-25000, France. s1franck@chu-besancon.fr.

Journal of Ovarian Research

|October 2, 2025

Summary

Multicolor flow cytometry (MFC) effectively characterizes ovarian tissue cell populations. This technique helps identify non-follicular cells that may improve ovarian function recovery after transplantation.

Keywords:

Fertility preservation Multicolor flow cytometry Ovarian tissue Ovarian tissue cryopreservation Qualification Stromal cell

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Last Updated: Jan 16, 2026

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Collection of Human Follicular Fluid, Follicle Somatic Cells, and Immature Oocytes from Individuals Undergoing In Vitro Fertilization

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Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles

Published on: November 30, 2022

Area of Science:

Reproductive Biology
Cell Biology
Tissue Engineering

Background:

Ovarian tissue autotransplantation is the sole method for utilizing cryopreserved ovarian tissue.
Graft quality, including follicle count and stromal environment, impacts revascularization and follicular development.
Limitations in graft quality hinder successful ovarian tissue transplantation.

Purpose of the Study:

To validate a technique for characterizing ovarian tissue.
To functionally qualify ovarian tissue for identifying key cell sub-populations.
To assess the potential of non-follicular cells in ovarian reconstruction.

Main Methods:

Ovarian cortex strips were obtained from women with polycystic ovary syndrome.
Fresh and cryopreserved ovarian tissues were dissociated.
Multicolor flow cytometry (MFC) analyzed cell yield, viability, and identified specific cell populations using antibodies.

Main Results:

Dissociation of fresh ovarian tissue yielded significantly more viable cells than frozen/thawed tissue.
Frozen/thawed ovarian tissue showed significantly higher viability post-dissociation compared to fresh tissue.
MFC identified distinct cell populations, including endothelial cells/progenitors, mesenchymal cells, and pericytes.

Conclusions:

MFC is an effective method for characterizing ovarian tissue cell populations.
Non-follicular cells may serve as prognostic factors for ovarian function recovery post-autotransplantation.
Identified cell populations could contribute to ovarian reconstruction programs.