Related Experiment Video
Updated: May 2, 2026
14:18
Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements
Published on: February 28, 2016
11.9K
Phase-Sensitive Surface Plasmon Resonance Imaging with Polarization Modulation and Stokes Vector Measurement
Baiqi Cui1, Xiaoyin Liu1, Jinbiao Ma1
1Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, P. R. China.
ACS Sensors
|October 2, 2025
Summary
A new Stokes vector-based polarization SPR imaging system offers enhanced sensitivity for biomolecular detection. This advanced phase-sensitive surface plasmon resonance (P-SPR) technique enables label-free quantification and multidimensional biosensing.
Area of Science:
- Biosensing and Nanophotonics
- Surface Plasmon Resonance (SPR) Technology
- Polarization Optics and Imaging
Background:
- Conventional intensity-based SPR methods lack the sensitivity of phase-sensitive techniques.
- Existing phase-sensitive SPR (P-SPR) implementations have limitations in optical complexity, phase resolution, and full polarization information access.
- There is a need for advanced P-SPR systems offering improved sensitivity and comprehensive data acquisition.
Purpose of the Study:
- To develop a compact and versatile Stokes vector-based polarization SPR imaging (Sp-SPRi) system.
- To overcome the limitations of current P-SPR techniques by enabling simultaneous acquisition of phase and multiple polarization parameters.
- To enhance molecular measurement capabilities and broaden the analytical scope of P-SPR.
Main Methods:
- Implementation of a Stokes vector-based polarization modulation and analysis for SPR imaging.
- Utilizing full Stokes vector analysis of imaging light to extract phase and polarization information.
- Development of a compact optical configuration with microfluidic integration for high-throughput imaging.
Main Results:
- Achieved a high phase sensitivity of 1.80 × 10-7 refractive index units (RIU).
- Demonstrated successful kinetic, label-free detection and quantification of biomolecular interactions.
- Validated performance through kinetic measurements of protein binding, small molecule interactions, and in situ glycoprotein analysis in single cells.
Conclusions:
- The Sp-SPRi system provides a powerful platform for multidimensional biosensing with simplified optics and high-throughput capabilities.
- This technique broadens the analytical scope of P-SPR, offering robust and accessible molecular measurement.
- The Sp-SPRi system holds significant potential for applications in point-of-care diagnostics, early disease detection, and drug screening.
Related Concept Videos
01:19Mass Analyzers: Common Types
2.0K
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
2.0K
01:21Tandem Mass Spectrometry
3.0K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
3.0K