Fiber laser based stimulated Raman photothermal microscopy towards a high-performance and user-friendly chemical...

Area of Science:

• Biophotonics
• Chemical Imaging
• Microscopy

Background:

• Stimulated Raman scattering (SRS) microscopy is a sensitive chemical imaging technique.
• Traditional SRS microscopy faces limitations due to bulky lasers and stringent sample requirements.
• High numerical aperture (NA) optics in SRS microscopy restrict sample types and imaging depth.

Purpose of the Study:

• To develop a compact and user-friendly fiber laser based stimulated Raman photothermal (SRP) microscope.
• To overcome the limitations of traditional SRS microscopy, including laser bulkiness and sample constraints.
• To enhance signal-to-noise ratio and imaging capabilities for diverse biological and chemical samples.

Main Methods:

• Development of a fiber laser system for stimulated Raman photothermal (SRP) microscopy.
• Implementation of low numerical aperture (NA), long working distance optics for signal collection.
• Utilizing urea for thermal enhancement and tissue clearance to improve sensitivity and imaging depth.

Main Results:

• Achieved a two-order-of-magnitude improvement in signal-to-noise ratio compared to fiber laser SRS without balance detection.
• Expanded sample compatibility from millimeters to centimeters, accommodating multi-well plates and thick tissues.
• Demonstrated enhanced sensitivity and imaging depth through the use of urea.

Conclusions:

• Fiber laser SRP microscopy presents a robust and portable alternative to traditional SRS microscopy.
• The developed platform addresses key limitations, offering improved performance and broader applicability.
• This technology provides a user-friendly solution for diverse chemical imaging applications.