Related Experiment Video

Updated: Jan 16, 2026

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.4K

Reprimo (RPRM): A Tumor Suppressor That Induces Extrinsic Apoptosis via YAP Signaling

Masahiro Takikawa1,2, Rieko Ohki1

  • 1Laboratory of Fundamental Oncology, National Cancer Center Research Institute, Tokyo, Japan.

Cancer Science
|October 2, 2025
PubMed

Insights

Reprimo (RPRM) is a tumor suppressor. It has a novel extracellular function, acting as a ligand that triggers apoptosis by regulating the Hippo-YAP pathway, offering new cancer therapy potential.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Signaling

Background:

  • Reprimo (RPRM) is a known tumor suppressor and p53 target gene.
  • RPRM promoter hypermethylation silences its transcription in various cancers.
  • Previous studies linked RPRM overexpression to cell cycle arrest, apoptosis, and DNA damage sensitivity.

Purpose of the Study:

  • To elucidate the complete molecular function of Reprimo.
  • To highlight Reprimo's novel extracellular role in inducing apoptosis.
  • To detail the newly identified p53-Reprimo-protocadherin-Hippo-YAP/TAZ-p73 signaling pathway.

Main Methods:

  • Literature review of Reprimo's known functions.
  • Analysis of recent studies on Reprimo's extracellular activity.
  • Examination of the novel signaling pathway involving Reprimo.

Main Results:

  • Reprimo is secreted extracellularly to induce apoptosis in target cells.
  • A novel signaling pathway (p53-Reprimo-protocadherin-Hippo-YAP/TAZ-p73) mediates this apoptosis.
  • Reprimo is the first identified extracellular ligand modulating YAP activity and regulating Hippo signaling.

Conclusions:

  • Reprimo possesses a unique extracellular tumor-suppressive mechanism.
  • Reprimo's role as an upstream regulator of Hippo signaling is significant.
  • Reprimo presents potential as a therapeutic target and agent in cancer treatment.
Keywords:
Reprimoapoptosishippo pathwayp53tumor suppressor

More Related Videos

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs10:32

Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Published on: December 19, 2019

11.5K
Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases06:56

Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases

Published on: September 6, 2024

804

Related Concept Videos

The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.3K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
8.1K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.7K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.2K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.3K