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Related Experiment Video

Updated: May 12, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
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Isometrically Resolved Reflection-Mode In Vivo Photoacoustic Microscopy Enabled by an Integrated Ultrasonic-Optical

Fan Yang1,2,3, Yong Yang2, Wei Song3

  • 1College of Optical and Electronic Technology, China Jiliang University, Hangzhou 310018, China.

ACS Sensors
|October 2, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed an Integrated Ultrasonic-Optical Sensor (IUOS) to improve photoacoustic microscopy. This novel sensor enhances depth localization accuracy for volumetric imaging, enabling clearer visualization of biological samples.

Keywords:
broad bandwidthin vivo imagingintegrated ultrasonic-optical sensorphotoacoustic microscopyspatial resolution

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Area of Science:

  • Biomedical Optics
  • Photoacoustic Imaging
  • Ultrasonic Transduction

Background:

  • Traditional photoacoustic microscopy struggles with depth localization accuracy due to limitations in ultrasonic transducers.
  • Narrow-bandwidth and optically opaque transducers hinder direct measurement of broadband photoacoustic waves.
  • These limitations compromise the precision of photoacoustic volumetric imaging.

Purpose of the Study:

  • To develop an Integrated Ultrasonic-Optical Sensor (IUOS) to overcome existing challenges in photoacoustic microscopy.
  • To enhance depth localization accuracy and enable high-resolution volumetric imaging.
  • To facilitate label-free in vivo visualization in biomedical research.

Main Methods:

  • Designed an Integrated Ultrasonic-Optical Sensor (IUOS) combining laser transmission and ultrasonic detection.
  • Utilized a nearly common-path Michelson interferometer configuration for signal transduction.
  • Integrated the IUOS into an optical-resolution photoacoustic microscope (OR-PAM) for reflection-mode imaging.

Main Results:

  • Achieved high sensitivity (127 Pa) over a broad bandwidth (158 MHz at -6 dB).
  • Enabled nearly lossless excitation laser transmission at 532 nm.
  • Demonstrated label-free in vivo volumetric visualization of zebrafish with improved depth localization.

Conclusions:

  • The IUOS significantly improves depth localization accuracy in photoacoustic volumetric imaging.
  • This sensor technology offers enhanced axial resolution for reflection-mode OR-PAM.
  • The IUOS holds considerable potential for advancing biomedical photoacoustic research and applications.