Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunomodulatory endothelial cells contribute to T cell recruitment and activation via antigen presentation on MHC II.

Cardiovascular research·2026
Same author

Antigenic characterization of SARS-CoV-2 variants BA.3.2.1 and BA.3.2.2 in three animal models.

bioRxiv : the preprint server for biology·2026
Same author

High-dose biologics and bioconjugates delivery: Integrating molecular optimization with device design and routes of administration.

Advanced drug delivery reviews·2026
Same author

Purine metabolic adaptation protects the endothelium from disturbed flow-induced DNA damage and atherosclerosis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Modeling early human heart development using an iPSC-based 3D bioprinted model of embryonic heart tube.

Nature communications·2026
Same author

Lymph Node Dissection and Radiation in the Rat Popliteal Region Leads to Progressive Lymphatic Pump Failure and Lymphedema.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jan 16, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

7.9K

Microfluidic Device Type Improves Heart mRNA Delivery In Vivo.

Elisa Schrader Echeverri1, Hyejin Kim1, Bora Jang1

  • 1Wallace H. Coulter Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, Georgia 30322, United States.

Langmuir : the ACS Journal of Surfaces and Colloids
|October 2, 2025
PubMed
Summary
This summary is machine-generated.

Formulation methods impact lipid nanoparticle (LNP) delivery. Using a herringbone mixer enhanced LNP heart delivery twofold compared to a bifurcating mixer, suggesting processing optimization for targeted delivery.

More Related Videos

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model
06:03

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

Published on: June 11, 2020

9.6K
A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice
06:42

A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice

Published on: April 16, 2018

17.0K

Related Experiment Videos

Last Updated: Jan 16, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

7.9K
Delivery of Modified mRNA in a Myocardial Infarction Mouse Model
06:03

Delivery of Modified mRNA in a Myocardial Infarction Mouse Model

Published on: June 11, 2020

9.6K
A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice
06:42

A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice

Published on: April 16, 2018

17.0K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Drug Delivery

Background:

  • Lipid nanoparticles (LNPs) are crucial for nucleic acid delivery.
  • Current strategies focus on LNP chemistry or targeting ligands to improve non-liver tissue delivery.
  • The formulation process remains an underexplored area for optimizing LNP delivery.

Purpose of the Study:

  • To investigate the impact of LNP formulation processes on tissue-specific delivery.
  • To compare the efficacy of LNPs formulated using different mixers for heart delivery.
  • To explore the mechanisms behind altered LNP biodistribution based on formulation.

Main Methods:

  • Formulation of LNPs using herringbone and bifurcating mixers.
  • Characterization of LNP biophysical properties.
  • Assessment of protein corona adsorption.
  • Spatial transcriptomics analysis of LNP delivery in a mouse model of atherosclerosis.
  • Quantification of LNP delivery to heart tissue and specific cell types.

Main Results:

  • LNPs formulated with a herringbone mixer showed a twofold increase in heart delivery compared to those from a bifurcating mixer.
  • Both LNP formulations exhibited similar biophysical characteristics.
  • Distinct protein coronas were observed on LNPs from different mixers, indicating formulation-dependent adsorption.
  • Spatial transcriptomics revealed targeted mRNA delivery to atherosclerotic regions and cardiomyocytes post-intravenous injection.

Conclusions:

  • The LNP formulation process significantly influences tissue-specific delivery, particularly to the heart.
  • Herringbone mixer-based formulation offers a promising strategy for enhanced cardiac LNP delivery.
  • Altered protein corona formation driven by formulation processing may mediate improved systemic delivery and targeting.