Investigating the prognostic role of citrullination-related genes in breast cancer by combining transcriptomics, single-cell analysis and verification
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View abstract on PubMed
Summary
This summary is machine-generated.This study identifies four key citrullination-related genes (CRGs) — SEZ6, S100B, SPIB, and TFF1 — that predict prognosis in breast cancer (BRCA). These genes also correlate with the tumor immune microenvironment, offering potential therapeutic targets.
Area Of Science
- Oncology
- Immunology
- Genetics
Background
- Citrullination is linked to autoimmune diseases and implicated in cancer, but its specific role in breast cancer (BRCA) prognosis is not well understood.
- Identifying prognostic biomarkers is crucial for improving breast cancer patient outcomes and developing targeted therapies.
Purpose Of The Study
- To identify citrullination-related genes (CRGs) that are associated with prognosis in breast cancer (BRCA).
- To develop a prognostic model based on identified CRGs and explore their relationship with the tumor immune microenvironment (TME).
Main Methods
- Utilized The Cancer Genome Atlas (TCGA-BRCA) and Gene Expression Omnibus (GEO) datasets to identify differentially expressed genes (DEGs) and intersect them with known CRGs.
- Employed univariate and Lasso Cox regression for CRG selection, followed by multivariate Cox regression to build a prognostic nomogram.
- Analyzed the correlation between CRGs, TME characteristics (e.g., immune cell infiltration, immune subtypes), and PD-L1 expression.
Main Results
- Identified SEZ6, S100B, SPIB, and TFF1 as key CRGs for constructing a prognostic risk model in BRCA.
- High-risk BRCA patients showed an immunosuppressive TME with low CD8+ T cell infiltration, while low-risk patients had higher immune infiltration.
- Specific CRGs correlated with distinct immune cell infiltration patterns (NK cells, T cells, neutrophils) and PD-L1 expression.
Conclusions
- SEZ6, S100B, SPIB, and TFF1 are significant prognostic genes in BRCA, offering insights into disease pathogenesis.
- The identified CRGs are associated with the tumor immune microenvironment, suggesting potential for immunomodulatory therapeutic strategies in BRCA.
- This study provides a foundation for developing personalized treatment strategies for breast cancer based on CRG expression and immune profiles.
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