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scRNA-seq of Penaeus japonicus hemocytes under environmentally-induced restriction of sand-diving behavior

  • 0Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, Jiangsu 222005, China.
Genomics +

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October 2, 2025

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October 2, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Restricting the sand-diving behavior of Penaeus japonicus shrimp induces a significant molecular stress response, particularly in granular and hyaline cells. This study identifies key genes involved in regulating this response to environmental changes.

Area Of Science

  • Marine Biology
  • Animal Behavior
  • Molecular Biology

Background

  • Penaeus japonicus is an economically vital shrimp species.
  • Sand-diving behavior in shrimp can limit aquaculture yield.
  • Understanding the molecular basis of behavioral responses is crucial for improving aquaculture practices.

Purpose Of The Study

  • To investigate the molecular stress response of Penaeus japonicus to the restriction of sand-diving behavior.
  • To identify genes and cell types involved in regulating burrowing behavior.

Main Methods

  • Single-cell RNA sequencing (scRNA-seq) to analyze gene expression differences across three culture systems (sandy, sandless, sandless stress).
  • Quantitative real-time PCR (qRT-PCR) to validate gene expression profiles.
  • RNA in situ hybridization to localize specific gene expression within cell types.

Main Results

  • Six hours post-transition to a sandless environment showed the most significant stress response.
  • Thirteen distinct cell subpopulations were identified, including granular cells (GCs), semi-granular cells (SGCs), and hyaline cells (HCs).
  • Differential gene expression analysis revealed significant changes, with GCs and HCs showing the most pronounced alterations, suggesting their role in burrowing behavior regulation.
  • Candidate genes such as trpa1, trpm, and cut protein family were identified as potentially playing roles in the response to environmental change.

Conclusions

  • The restriction of sand-diving behavior in Penaeus japonicus triggers a notable molecular stress response.
  • Granular cells and hyaline cells are key players in the regulation of burrowing behavior.
  • Identified candidate genes provide a foundation for future research into behavioral regulation and aquaculture improvement in shrimp.

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