Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

1.4K
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
1.4K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.3K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.3K
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

1.6K
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
1.6K
Mass Spectrometers01:16

Mass Spectrometers

8.3K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
8.3K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.6K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.6K
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

2.7K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Elucidating Protein Quaternary Structure in Near-Native Solution: An AI-Assisted Hydrodynamic Approach.

Analytical chemistry·2026
Same author

Multi-Omics Analysis Identifies Paxillin as a Biomarker of Doxorubicin Resistance via Cytoskeletal Remodeling and Immune Exhaustion.

Journal of proteome research·2026
Same author

Integrated Transcriptomic and Proteomic Analyses Reveal Stage-Dependent Gill Remodeling Under Chronic Salinity Stress in Grass Carp (Ctenopharyngodon idella).

Marine biotechnology (New York, N.Y.)·2026
Same author

Rapid On-Site Analysis of Psychotropic Drugs in Dried Blood Spots by Capillary-in-Capillary Electrospray Ionization (DBS-CC-ESI) Miniature Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

FPM-DCN: Fourier ptychographic microscopy with a dual-constrained network for robust reconstruction in complex conditions.

Optics express·2026
Same author

Minimalist optical system for achromatic imaging within extended field of view based on monolithic integrated meta-axicon cluster.

Light, science & applications·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
Same journal

Development of a Tapping-Mode Scanning Probe Electrospray Ionization Platform for High-Sensitivity and Long-Term Stability in Single-Cell Mass Spectrometry Imaging of Tissue.

Analytical chemistry·2026
Same journal

A Solid-State Near-Infrared Fluorescent Probe by a Synergistic Extended Conjugated System for Detecting Cys with Long-Term Imaging in Orthotopic Bladder Cancer.

Analytical chemistry·2026
Same journal

Air-Based Porous Array Dielectric Barrier Discharge Ionization Source for Explosive Trace Detection.

Analytical chemistry·2026
Same journal

PSAQ<sup>+1</sup>: Absolute Protein Quantification Using a <sup>13</sup>C<sub>1</sub>-Labeled Protein Standard, Coisolation of Peptide Pairs and LC-PRM.

Analytical chemistry·2026
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

14.9K

Dual-Trap "Brick" Miniature Mass Spectrometer with Enhanced Sensitivity and Fragmentation Capabilities.

Ronghui Ma1, Yongguang Han2, Ting Jiang1

  • 1School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China.

Analytical Chemistry
|October 2, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel dual-trap miniature mass spectrometer (mini-MS) that significantly enhances sensitivity and analytical capabilities for on-site analysis. The innovative design achieves a ~20-fold sensitivity boost, enabling high-performance field applications.

More Related Videos

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

24.8K
Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
07:53

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry

Published on: March 1, 2020

7.8K

Related Experiment Videos

Last Updated: Jan 16, 2026

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

14.9K
Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

24.8K
Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
07:53

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry

Published on: March 1, 2020

7.8K

Area of Science:

  • Analytical Chemistry
  • Instrument Development
  • Mass Spectrometry

Background:

  • Miniaturization of mass spectrometry (MS) often limits analytical performance.
  • On-site analysis of complex samples demands enhanced capabilities from miniature mass spectrometers (mini-MS).

Purpose of the Study:

  • To develop a next-generation mini-MS with improved performance for field-deployable applications.
  • To overcome the limitations of current mini-MS technology through innovative design.

Main Methods:

  • Development of a "Brick" mini-MS featuring a differential-pressure dual-trap configuration.
  • Implementation of parallel ion accumulation/cooling at higher pressures and ion isolation/MS analysis at lower pressures.
  • Investigation of various ion fragmentation techniques, including collision-induced dissociation (CID), transfer dissociation, and high-pressure collisional dissociation (HpCD).

Main Results:

  • The dual-trap configuration enables parallel ion manipulation and diverse fragmentation modes.
  • Parallel ion accumulation mode increased sensitivity by approximately 20-fold, reaching 50 pg/mL.
  • High-pressure collisional dissociation (HpCD) generated extensive ion fragments comparable to beam-type collisional activation dissociation methods.

Conclusions:

  • The developed dual-trap mini-MS significantly advances the capabilities of miniature mass spectrometry.
  • This enhanced mini-MS is suitable for high-performance, field-deployable analytical tasks.
  • The novel design overcomes previous limitations, paving the way for more powerful portable analytical instruments.