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Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

3.5K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
3.5K
Atomic Spectroscopy: Absorption, Emission, and Fluorescence01:23

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

2.6K
Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
2.6K
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

568
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
568
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

665
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
665
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

4.7K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
4.7K
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

1.2K
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
1.2K

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Related Experiment Video

Updated: Jan 16, 2026

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering
07:55

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

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Enzymatic X-ray absorption spectroelectrochemistry.

Rafael N P Colombo1, Graziela C Sedenho1, Itamar T Neckel2

  • 1São Carlos Institute of Chemistry, University of São Paulo, São Carlos, Brazil.

Nature Protocols
|October 2, 2025
PubMed
Summary
This summary is machine-generated.

Enzymatic X-ray absorption spectroelectrochemistry (XA-SEC) enables detailed study of protein redox properties and catalysis. This method combines X-ray spectroscopy with electrochemistry for advanced bio-inspired catalyst design.

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Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

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Area of Science:

  • Biochemistry
  • Electrochemistry
  • Spectroscopy

Background:

  • Understanding protein redox properties is crucial for biocatalysis and designing bio-inspired catalysts.
  • Enzymatic X-ray absorption spectroelectrochemistry (XA-SEC) integrates X-ray absorption spectroscopy (XAS) and electrochemical methods to study enzyme redox behavior.

Purpose of the Study:

  • To describe a protocol for performing enzymatic XA-SEC experiments.
  • To demonstrate efficient enzyme immobilization on carbon electrodes using nanomaterials.
  • To provide insights into enzymatic electrocatalysis for developing sustainable bioelectrochemical technologies.

Main Methods:

  • Enzyme immobilization on carbon-based electrodes, exemplified by bilirubin oxidase.
  • Setting up a three-electrode electrochemical cell with proper connections and electrolyte preparation.
  • Cu K-edge X-ray absorption spectroscopy measurements at synchrotron light sources with in situ electrochemical control.

Main Results:

  • Successful immobilization of bilirubin oxidase using nanomaterials for enhanced loading and electron transfer.
  • Stable electrochemical and spectroscopic signals during long experimental runs, indicating protein stability under X-ray exposure.
  • Real-time monitoring of redox processes via direct electron transfer analysis, yielding thermodynamic and kinetic information.

Conclusions:

  • Enzymatic XA-SEC is a powerful tool for understanding enzymatic electrocatalysis.
  • The described protocol facilitates the study of enzyme redox properties and catalytic behavior.
  • This method advances the development of sustainable bioelectrochemical technologies.