Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

21.5K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
21.5K
CRISPR01:59

CRISPR

57.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.5K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Repeatability of SpotChecks contrast sensitivity test in macular disease.

Optometry and vision science : official publication of the American Academy of Optometry·2026
Same author

Development and Assessment of Rutin/Betamethasone-Loaded PAN-PVP Nanofibers for Wound Healing Applications.

Current drug delivery·2026
Same author

High-Spin Pt Sites of Intermetallic Compound via Pinning Effect Boost Oxygen Reduction Performance.

Angewandte Chemie (International ed. in English)·2026
Same author

Atomically Engineered RuO<sub>x</sub>-Cu Interfaces Enabling Tandem Catalysis for Ampere-Level Nitrite-Ethanol Co-Electrolysis.

Angewandte Chemie (International ed. in English)·2026
Same author

Superfine grinding on the physicochemical properties, volatile compounds, and bioactive properties of <i>Forsythia suspensa</i> (Thunb.) Vahl fruit powder.

Food chemistry: X·2026
Same author

Molecular epidemiology and evolutionary analysis of multidrug-resistant Acinetobacter baumannii ST<sup>oxf</sup>2499 clone at a tertiary hospital in Tianjin, China.

Journal of global antimicrobial resistance·2026
Same journal

Natural Variation and Expression of OsCFF1 Affect the Formation of Rice Chalkiness.

Rice (New York, N.Y.)·2026
Same journal

OsWRKY11 Regulates Rice Leaf Angle Via Transcriptional Activation of OsGSR1.

Rice (New York, N.Y.)·2026
Same journal

CRISPR/Cas9-Mediated Mutagenesis of OsERF94 Enhances Pre-Harvest Sprouting in Rice.

Rice (New York, N.Y.)·2026
Same journal

Knockout of OsRbohD (the NADPH Oxidase Gene) Enhances Saline-Alkaline Stress Tolerance and Grain Yield in Rice by Reducing ROS Accumulation.

Rice (New York, N.Y.)·2026
Same journal

OsHOX24 Modulates Grain Size and Salt Tolerance Via OsPP2C09 in Rice.

Rice (New York, N.Y.)·2026
Same journal

Morphological Characteristics and Significance of Bulliform Phytoliths in Haploid Rice.

Rice (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K

Seeing Red, Selecting True: RUBY-Reported Seed Marker Streamlines CRISPR-Clean Rice Breeding.

Jin-Lei Liu1,2,3, Tao Yang1,3, Yu-Wei Fu4

  • 1State Key Laboratory of Forage Breeding-by-Design and Utilization, Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

Rice (New York, N.Y.)
|October 3, 2025
PubMed
Summary
This summary is machine-generated.

We developed a new CRISPR-Cas9 toolkit for plants, using a visual marker to easily identify edited offspring. This method simplifies creating genetically stable, transgene-free plants for research.

Area of Science:

  • Plant Genomics
  • Gene Editing Technologies
  • Molecular Biology

Background:

  • CRISPR-Cas9 is crucial for plant gene editing, but removing the editing construct is essential for stable, genetically modified plants.
Keywords:
CRISPR-Cas9Genome editingRUBYTransgene-freeVisible marker-assisted selection

More Related Videos

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

13.2K
Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

7.7K

Related Experiment Videos

Last Updated: Jan 16, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.3K
Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

13.2K
Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

7.7K
  • Current methods for removing CRISPR constructs are often complex and not universally applicable.