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Overview of Secretory Vesicles01:33

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Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
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Celery root-plant derived vesicles: comprehensive isolation, characterization and proteomic analysis.

Ezgi Taşkan1, Oğuz Kaan Kırbaş1, Derya Sağraç1

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Celery root-derived plant-derived vesicles (CR-PDVs) were characterized for the first time. These novel nanocarriers show potential for disease modeling and therapeutic applications.

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Area of Science:

  • Biotechnology
  • Nanomedicine
  • Plant Science

Background:

  • Celery root (Apium graveolens) possesses antioxidant and anti-inflammatory properties but remains understudied compared to its leaves.
  • Plant-derived vesicles (PDVs) are biocompatible nanocarriers with emerging biotechnological applications.
  • Comprehensive characterization of celery root-derived PDVs (CR-PDVs) is lacking in scientific literature.

Purpose of the Study:

  • To conduct the first comprehensive physical and proteomic characterization of CR-PDVs.
  • To explore the potential of CR-PDVs in disease modeling and therapeutic strategies.

Main Methods:

  • Purification of CR-PDVs using the Aqueous Two-Phase System (ATPS) protocol.
  • Analysis of morphology, size distribution, and particle density via Nanoparticle Tracking Analysis (NTA).
  • Proteomic profiling using LC-MS/MS, followed by Gene Ontology and KEGG enrichment analyses for functional classification.

Main Results:

  • CR-PDVs were identified as homogeneous spherical structures with a modal diameter of 147.6 ± 3 nm.
  • A total of 168 proteins were identified, primarily involved in folding, chaperoning, vesicle biogenesis, and oxidative stress response.
  • KEGG analysis highlighted prominent proteasome, ribosome, and phagosome pathways, indicating roles in protein synthesis, degradation, and cellular uptake.

Conclusions:

  • CR-PDVs exhibit high structural integrity and a rich cargo, making them promising candidates for drug delivery and biomarker platforms.
  • These findings establish a foundational dataset for CR-PDVs, supporting their evaluation in disease models such as cancer, neurodegenerative, and autoimmune disorders.
  • This research paves the way for translational applications of CR-PDVs in medicine.