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Updated: Aug 4, 2026
Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
Production of antibody to staphylococcal delta-haemolysin in the rabbit
Abstract:
Gamma-globulin and IgG from the sera of rabbits immunized with either "insoluble" or "solvent-transferred" staphylococcal delta-haemolysin precipitated with, and neutralized both forms of lysin. Similar preparations from the same rabbits before immunization did not; nor did those from rabbits made hyperimmune to other antigens. In these tests "insoluble" and "solvent-transferred" lysin could not be distinguished antigenically, nor could "insoluble" lysin from two different strains of staphylococci, each grown on two different media. An appendix presents further evidence for antigenicity of delta-lysin, based on affinity chromatography.
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