Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mucinase-engineered cell membrane nanovesicles degrade the glycocalyx shield to potentiate antitumor immunity.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Photoactivated Ruthenium-Platinum Complex Enhances Immune Checkpoint Blockade Therapy by Upregulating MHC-I Expression and Inducing Immunogenic Cell Death.

Journal of medicinal chemistry·2026
Same author

Inert Complexes Unlock Ligand-Accelerated Transition-Metal Catalysis on Proteins.

Angewandte Chemie (International ed. in English)·2026
Same author

Targeted stress granule regulation by engineering a non-catalytic O-GlcNAc transferase.

Nature communications·2026
Same author

Rapid MS-Based Single-Cell Analysis Assisted by Photochemical Derivatization in Living Cell Membranes Reveals Lipidomic Patterns with Located C═C Bonds.

Analytical chemistry·2025
Same author

A biparatopic HER2-targeting ADC constructed <i>via</i> site-specific glycan conjugation exhibits superior stability, safety, and efficacy.

RSC chemical biology·2025

Related Experiment Video

Updated: Jan 16, 2026

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
07:34

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

Published on: June 2, 2011

25.3K

Cocktail Chemical Labeling for In-Depth Surfaceome Profiling of Bone-Marrow-Derived Dendritic Cells.

Shiming Sun1,2, Siyang Liu3, Tao Deng2

  • 1Institute of Chemical Biology, Shenzhen Bay Laboratory, Shenzhen 518132, China.

Analytical Chemistry
|October 3, 2025
PubMed
Summary

Researchers developed a new method using [Ru(bpy)3]Cl2 (Ru) for efficient cell surface protein labeling. This technique offers deep surfaceome coverage for identifying cell types and states, aiding in drug target discovery.

More Related Videos

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

1.8K
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
08:01

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

12.9K

Related Experiment Videos

Last Updated: Jan 16, 2026

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
07:34

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

Published on: June 2, 2011

25.3K
Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

1.8K
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
08:01

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

12.9K

Area of Science:

  • Proteomics
  • Cell Biology
  • Biochemistry

Background:

  • Cell surface proteins (CSPs) are vital biomarkers for cell identification and state determination, particularly in dendritic cells (DCs).
  • Existing proteomic methods for CSP profiling face limitations due to protein hydrophobicity, low abundance, and the need for genetic manipulation, leading to biased and inefficient labeling.

Purpose of the Study:

  • To develop a novel, efficient, and user-friendly method for comprehensive cell surface protein profiling.
  • To overcome the limitations of current proteomic techniques for cell surfaceome analysis.

Main Methods:

  • Utilized [Ru(bpy)3]Cl2 (Ru) for effective cell surface biotinylation via a simple "mix and lighten" photoredox approach.
  • Employed a versatile probe cocktail of biotin-phenol and biotin-hydrazide to enhance substrate coverage.
  • Applied the method to HeLa cells and primary bone-marrow-derived dendritic cells undergoing differentiation.

Main Results:

  • Achieved reproducible identification of up to 733 plasma membrane proteins on HeLa cells.
  • Successfully mapped dynamic changes in the surfaceome during dendritic cell differentiation.
  • Demonstrated a user-friendly tool for deep surfaceome coverage and profiling dynamic changes in primary cells.

Conclusions:

  • The Ru-catalyzed "mix and lighten" method provides a powerful and efficient tool for cell surfaceome analysis.
  • This approach has significant potential for understanding cell identities, functional states, and identifying novel therapeutic targets.