Determining the type of aerobic/anaerobic metabolism during exercise in Drosophila using incremental loading exercise

Currently, Drosophila exercise is only classified as regular or endurance exercise, and there is no aerobic or anaerobic form of exercise, making Drosophila exercise modeling studies limited. Therefore, this study aimed to determine the type of aerobic and anaerobic exercise in Drosophila using Incremental Load exercise. A seven-day-old wild-type Drosophila was used as a study sample for exercise intervention using a Drosophila exercise device. This study was designed with six exercise intensities, including 0.31 rev/s (E1), 0.45 rev/s (E2), 0.59 rev/s (E3), 0.71 rev/s (E4), 0.83 rev/s (E5), 0.91 rev/s (E6), and Control in addition Drosophila in all exercise groups performed a one-time 2.5-h acute exercise, and the control group did no exercise intervention. Anaerobic and aerobic metabolic enzymes in Drosophila were assayed immediately after exercise. Additionally, Drosophila exercise types were determined using hemolymph lactate, respiratory quotient, trehalose concentration, and mitochondrial respiration assays. The experimental results show that at E2 exercise intensity, Drosophila mainly uses aerobic metabolism, and at E6 exercise intensity, it mainly uses anaerobic metabolism.We exercised Drosophila for 2 weeks at E2 and E6 exercise intensities to examine whether the physiological differences (cardiac function, climbing ability, sleep ability, lifespan, etc.) were consistent with those observed in mammals undergoing long-term aerobic and anaerobic exercise.The results showed that these differences are consistent with mammals.