Discovery of iridoid cyclase completes the iridoid pathway in asterids
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View abstract on PubMed
Summary
This summary is machine-generated.Researchers discovered iridoid cyclases, enzymes crucial for iridoid biosynthesis in plants. This finding unlocks new pathways for producing valuable iridoid compounds sustainably.
Area Of Science
- Plant biochemistry
- Metabolic engineering
- Molecular biology
Background
- Iridoids are vital plant compounds derived from monoterpenes, essential for defense and alkaloid precursor synthesis in asterids.
- The early iridoid biosynthesis pathway, involving 8-oxocitronellyl enol cyclization, was well-studied but had missing enzymatic steps.
Purpose Of The Study
- To identify and characterize the iridoid cyclases responsible for a key cyclization step in iridoid biosynthesis.
- To explore the phylogenetic distribution of these enzymes across diverse asterid species.
Main Methods
- Phylogenetic analysis of iridoid-producing asterid species.
- Single-nuclei sequencing to identify candidate genes.
- Enzymatic assays to confirm catalytic activity of discovered iridoid cyclases.
Main Results
- Discovery of iridoid cyclases from a broad range of asterid species.
- Demonstration that these enzymes catalyze the formation of 7S-cis-trans and 7R-cis-cis nepetalactol.
- Identification of a previously missing enzymatic step in early iridoid biosynthesis.
Conclusions
- The identified iridoid cyclases represent a key missing link in asterid iridoid biosynthesis.
- This discovery enables the production of novel iridoid stereoisomers.
- Facilitates metabolic engineering for sustainable production of iridoids and their derivatives.
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