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Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
Published on: June 9, 2023
CircRNA-14052 promotes breast cancer progression via miR-214-3p/IKBKB pathway
Donghai Li1, Zhiying Zhang1, Yan Cui2
1Department of Thyroid Breast Surgery, Affiliated Hospital of Inner Mongolia Medical University, Hohhot North Street, Hohhot, 010050, Inner Mongolia, China.
Objectives:
Circular RNAs play crucial regulatory roles in the progression of human diseases. This study aimed to investigate the functional mechanism of circRNA-14,052 in breast cancer progression.
Methods:
The biological functions of circRNA-14,052 were assessed using CCK-8, wound healing, flow cytometry assays. The ceRNA regulatory network of circRNA-14,052-miR-214-3p- IKBKB was validated by luciferase reporter assay.
Results:
The levels of circRNA-14,052 were notably elevated, but the levels of miR-214-3p were markedly reduced in breast cancer tissues compared to adjacent non-cancerous tissues. Downregulation of circRNA-14,052 or overexpression of miR-214-3p reduced MCF-7 cell proliferation and triggered cell apoptosis. Mechanically, circRNA-14,052 could elevate IKBKB levels via competitively sponging miR-214-3p. Notably, miR-214-3p inhibition reversed the growth-suppressive effects of circRNA-14,052 silencing. Additionally, circRNA-14,052 knockdown notably reduced IKBKB, IL-6, JAK2 and STAT3 levels in MCF-7 cells; whereas these changes were reversed by miR-214-3p deficiency. Furthermore, deficiency of circRNA-14,052 reduced xenograft tumor growth in vivo through targeting miR-214-3p/IKBKB/IL-6/JAK2/STAT3 axis.
Conclusion:
Collectively, our results showed that circRNA-14,052 promotes breast cancer progression via the miR-214-3p/IKBKB axis. Targeting this molecular axis may represent a promising therapeutic strategy for breast cancer treatment.
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