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α-hederin inhibits cervical cancer progression by inducing DNA damage-dependent cell cycle blockade and apoptosis

Yanlun Song1,2,3, Haimei Qin1,2,3, Shaofeng Huang3,4

  • 1Reproductive Medicine Center, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China.

BMC Cancer
|October 4, 2025
PubMed
Abstract

Insights

Alpha-hederin shows promise for treating cervical cancer by inhibiting cell growth and promoting apoptosis. This compound effectively targets cancer cell proliferation and progression in both lab studies and animal models.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Cervical cancer incidence remains high despite HPV vaccination and screening.
  • There is a need for novel therapeutic strategies against cervical cancer.

Purpose of the Study:

  • To investigate the anti-cancer potential of α-hederin in cervical cancer.
  • To elucidate the molecular mechanisms underlying α-hederin's effects on cervical cancer cells.

Main Methods:

  • Cell viability, apoptosis, cell cycle, ROS, and mitochondrial membrane potential assays.
  • Transcriptome sequencing, bioinformatics, and single-cell sequencing for mechanism exploration.
  • Western blot, immunofluorescence, gene overexpression, and in vivo tumorigenicity experiments in nude mice.

Main Results:

  • α-hederin inhibited cervical cancer cell growth, migration, and invasion while promoting apoptosis.
  • The compound regulated cell cycle and DNA replication pathways, specifically inhibiting the G2M phase by suppressing CDK1 and CyclinB expression.
  • In vivo studies confirmed α-hederin's efficacy in inhibiting cervical cancer growth.

Conclusions:

  • α-hederin demonstrates significant potential as an effective therapeutic agent for cervical cancer.
  • Further development of α-hederin for cervical cancer treatment is warranted due to its promising prospects.
Keywords:
Cell cycleCervical cancerDNA damageα-hederin

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