Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Spatial analysis identifies LAMTOR2 overexpression in hepatocellular carcinoma with vessels encapsulating tumor clusters.

Hepatology international·2026

Same author

Integrative Single-Cell and Spatial Transcriptomic Analysis Reveals MAIT Cell Dysfunction in Relapsed HCC.

Journal of hepatocellular carcinoma·2026

Same author

Type 2 lymphocytes restrict type 3 lymphocytes during liver fibrosis and colocalize in fibroblast niches.

Science advances·2026

Same author

RFC1 Repeat Expansion in Chronic Cough: Findings From the Korean Chronic Cough Registry.

Allergy, asthma & immunology research·2026

Same author

Molecular Improvement of IL-23 Inhibition Revealed by Single-Cell RNA Sequencing in Concurrent Psoriasis and Systemic Lupus Erythematosus.

Experimental dermatology·2026

Same author

Aging Mediates Inflammatory Transformation of Kidney-Resident Macrophages via Thrombospondin-1 Signaling.

Immune network·2026

Search research articles

Related Experiment Video

Updated: Jan 16, 2026

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

Single-Cell Transcriptomic Profiling Reveals a Distinct Monocyte MIF/ANXA1 Signature Associated With Poor

Haeyoon Kwon¹, Minji Kang², Soyoung Jeong²

¹Department of Medicine, Seoul National University College of Medicine, Seoul, Korea.

Allergy, Asthma & Immunology Research

|October 4, 2025

Summary

Inhaled corticosteroids (ICSs) are crucial for asthma, but some patients don't respond well. This study reveals that dysregulation of MIF and ANXA1 in monocytes is a key immune signature linked to poor ICS response in asthma patients.

Keywords:

Asthma annexin A1 formyl peptide receptor glucocorticoids macrophage migration inhibitory factor monocytes single-cell transcriptome analysis treatment effect heterogeneity

More Related Videos

On-Chip Endothelial Inflammatory Phenotyping

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Published on: October 14, 2021

Related Experiment Videos

Last Updated: Jan 16, 2026

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Published on: October 17, 2017

On-Chip Endothelial Inflammatory Phenotyping

On-Chip Endothelial Inflammatory Phenotyping

Published on: July 21, 2012

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Published on: October 14, 2021

Area of Science:

Immunology
Pulmonology
Genomics

Background:

Inhaled corticosteroids (ICSs) are primary asthma treatments.
A subset of asthma patients shows poor response to ICS therapy.
Understanding treatment heterogeneity is vital for targeted therapies.

Purpose of the Study:

To investigate the cellular and molecular mechanisms behind variable ICS response in asthma.
To identify immune cell subsets and signaling pathways associated with ICS non-response.

Main Methods:

Single-cell RNA sequencing of peripheral blood mononuclear cells from healthy controls, ICS responders, and ICS poor responders.
Differential gene expression and receptor-ligand interaction analysis.
Focus on CD14+ monocyte transcriptional profiles.

Main Results:

ICS poor responders had fewer circulating CD14+ monocytes.
Chemotaxis-related genes (CCR1, CCL2, CCL7, CXCL2) were upregulated in poor responders.
ANXA1/FPR2 were downregulated, while MIF/receptors were upregulated in poor responders, with T cells identified as a potential MIF source.

Conclusions:

MIF-ANXA1 pathway dysregulation in CD14+ monocytes is a significant immune signature in asthma patients with poor ICS response.
This finding may inform the development of targeted therapies for non-responders.