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Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

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A new H₂Se-selective fluorescent probe based on nitro reduction.

Wenfang Liang¹, Liyue Wang², Zhili Yu³

¹College of Chemical Engineering, Beijing University of Chemical Technology (BUCT), Beijing 100029, China. yilong@mail.buct.edu.cn.

Organic & Biomolecular Chemistry

|October 4, 2025

Summary

This summary is machine-generated.

Researchers developed a new probe, Nap-NO2-mCl, for highly selective detection of hydrogen selenide (H2Se) in biological systems. This tool enables effective monitoring of H2Se in living cells.

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Area of Science:

Biochemistry
Chemical Biology
Molecular Biology

Background:

Hydrogen selenide (H2Se) is recognized as a bioactive molecule involved in various physiological and pathological conditions.
Accurate detection of H2Se is crucial for understanding its biological roles.

Purpose of the Study:

To develop a highly selective and sensitive detection tool for hydrogen selenide (H2Se).
To investigate the utility of the developed probe in monitoring H2Se in biological contexts.

Main Methods:

Rational design of H2Se probes utilizing nitroaromatic reduction and self-immolative processes.
Synthesis and characterization of the Nap-NO2-mCl probe.
Evaluation of probe selectivity, sensitivity, and performance in detecting H2Se in living cells.

Main Results:

A novel H2Se detection platform, Nap-NO2-mCl, was successfully developed.
The Nap-NO2-mCl probe demonstrated high selectivity and sensitivity towards H2Se.
The probe effectively monitored both triggered-release and exogenous H2Se in living cells.

Conclusions:

The Nap-NO2-mCl probe represents a valuable tool for H2Se detection in biological research.
This platform facilitates further investigation into the physiological and pathological functions of H2Se.