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Functional characterization of TaNAC6-3B: A key regulator of drought tolerance in wheat (Triticum aestivum L.)
Nan Chen1, Xiang Li1, Yong-Jia Feng2
1State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, P. R. China.
Abstract:
Drought stress is a major abiotic constraint limiting wheat (Triticum aestivum L.) productivity. Previous studies have shown that reducing irrigation water use by approximately 40 % can cause a 20.6 % decrease in wheat yield. Therefore, improving drought resistance is a priority in wheat breeding programs. For genetic improvement of drought tolerance, systematic investigation of drought-responsive molecular mechanisms is crucial. In this study, comparative transcriptome analysis was conducted on leaf and root tissues of the drought-tolerant wheat cultivar ChangWu134 under well-watered and drought-stressed conditions. Further systematic analysis identified a key drought tolerance gene TaNAC6-3B. Functional characterization of the candidate NAC (NAM, ATAF1/2, and CUC2) family transcription factor TaNAC6-3B revealed its nuclear localization. Transgenic overexpression lines had significantly enhanced drought tolerance, and transcriptome profiling revealed up-regulation of NCED and ABA responsive genes and drought-responsive genes. Mechanism studies have revealed that TaNAC6-3B activates the expression of the LEA (Late embryogenesis abundant) protein gene TaLEA1-2B via direct binding to its promoter. The results of this study provide clues for analysis of the genetic basis of drought tolerance in ChangWu134, and also provide candidate genetic resources for breeding for drought tolerance.
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