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Updated: Jan 16, 2026

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
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Salmonella typhimurium downregulates circHIPK2 expression via CeRNA mechanism to inhibit cell migration and

Fei Wang1,2, Xiaoting Luan2, Huihui Ma2

  • 1The Affiliated Mental Health Center of Jiangnan University, Wuxi Central Rehabilitation Hospital, Wuxi, 214151, Jiangsu, China.

Gut Pathogens
|October 4, 2025
PubMed
Abstract

Insights

Salmonella typhimurium infection alters circular RNA (circRNA) expression in colorectal cancer (CRC). The circHIPK2/miR-124-3p pathway is key in CRC pathogenesis and a potential therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Microbiology

Background:

  • Salmonella typhimurium is a common intestinal pathogen.
  • Circular RNAs (circRNAs) are critical in human disease pathogenesis.
  • The role of host circRNAs in S. typhimurium infection during colorectal cancer (CRC) development is unclear.

Purpose of the Study:

  • To investigate the therapeutic potential of altered circRNA expression in S. typhimurium-infected CRC.
  • To elucidate the mechanisms of circRNA regulation by S. typhimurium in CRC.

Main Methods:

  • Transcriptomic analysis of HCT116 cells infected with S. typhimurium.
  • circHIPK2 knockdown and overexpression experiments.
  • RNA molecular binding assays to confirm interactions.

Main Results:

  • S. typhimurium significantly altered cellular circRNA expression in HCT116 cells.
  • circHIPK2 was notably downregulated and its knockdown inhibited cell motility, tumor growth, and cytokine expression.
  • circHIPK2 interacts with miR-124-3p, and miR-124-3p overexpression reversed circHIPK2-induced effects on cell motility.

Conclusions:

  • The S. typhimurium-regulated circHIPK2/miR-124-3p axis is crucial in CRC pathogenesis.
  • This axis represents a promising molecular therapeutic target for colitis-associated CRC.
Keywords:
Salmonella typhimuriumCircHIPK2Colorectal cancerMir-124-3p

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