Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.1K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.1K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.4K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal characterization of articular cartilage degeneration in the humeral head using Raman spectroscopy, biomechanics, and imaging.

Osteoarthritis and cartilage·2026
Same author

Comparative analysis of the GRAS transcription factor family in oilseed and confectionery sunflower identifies HaGRAS19 as a regulator of salt stress responses.

Plant physiology and biochemistry : PPB·2026
Same author

Polarization-engineered aberration-resilient light sheet microscopy.

bioRxiv : the preprint server for biology·2026
Same author

Intraoperative Hip Arthrography to Guide Decision-Making in Cerebral Palsy Hip Reconstruction.

Journal of pediatric orthopedics·2026
Same author

Prenatal per- and polyfluoroalkyl substances exposure and child neurodevelopment from birth to age 7: A prospective study in the Laizhou Wan Birth Cohort.

Journal of hazardous materials·2026
Same author

Complete genome sequence of <i>Sedimentibacter</i> sp. strain MB31-C6, isolated from sewage sludge.

Microbiology resource announcements·2026
Same journal

Dual-Modal Phototherapeutic Nanoagents Eradicating Drug-Resistant Bacteria via Multi-Pathway of Membrane Disruption, Oxidative Damage, and Energy Metabolism Interference.

Advanced healthcare materials·2026
Same journal

Smartphone-Enabled Point-of-Care Biosensing Platform With Self-Calibration for Rapid Matrix-Resistant Detection of Multiple AMI Biomarkers in Whole Blood.

Advanced healthcare materials·2026
Same journal

Multimetal-Doped Nanoenzymes Reprogram Macrophages for Immunotherapy of Gouty Arthritis.

Advanced healthcare materials·2026
Same journal

Correction to "Fibrosis-on-Chip: A Guide to Recapitulate the Essential Features of Fibrotic Disease".

Advanced healthcare materials·2026
Same journal

A Collagen-based Scaffold Supports Tendon-to-bone Healing After Rotator Cuff Repair: An Integrated Translational Study.

Advanced healthcare materials·2026
Same journal

A Biomimetic Copper-Caffeic Acid Nanozyme Activates Cuproptosis and Pyroptosis by Mimicking the Neutrophil Enzymatic Cascade.

Advanced healthcare materials·2026
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research
07:29

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research

Published on: September 27, 2024

1.2K

Evaluation of Engineered Cartilage Composition and Function Using Raman Spectroscopy.

Dev R Mehrotra1, Carolina V Cordova1, Tianbai Wang2

  • 1Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA, 02215, USA.

Advanced Healthcare Materials
|October 6, 2025
PubMed
Summary
This summary is machine-generated.

Raman spectroscopy non-destructively monitors tissue-engineered cartilage (TEC) composition and mechanical properties. This platform optimizes cartilage regeneration therapies by analyzing extracellular matrix (ECM) biomarkers.

Keywords:
cartilage tissue engineeringnondestructive testingraman spectroscopytissue‐engineered medical product

More Related Videos

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.5K
Biotribological Testing and Analysis of Articular Cartilage Sliding against Metal for Implants
09:08

Biotribological Testing and Analysis of Articular Cartilage Sliding against Metal for Implants

Published on: May 14, 2020

4.2K

Related Experiment Videos

Last Updated: Jan 16, 2026

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research
07:29

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research

Published on: September 27, 2024

1.2K
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

17.5K
Biotribological Testing and Analysis of Articular Cartilage Sliding against Metal for Implants
09:08

Biotribological Testing and Analysis of Articular Cartilage Sliding against Metal for Implants

Published on: May 14, 2020

4.2K

Area of Science:

  • Biomedical Engineering
  • Spectroscopy
  • Regenerative Medicine

Background:

  • Tissue-engineered cartilage (TEC) development requires methods to monitor composition and mechanical properties.
  • Raman spectroscopy offers a non-destructive technique to analyze biochemical composition.

Purpose of the Study:

  • To develop and validate a Raman spectroscopy-based platform for monitoring TEC composition.
  • To correlate spectroscopic biomarkers with extracellular matrix (ECM) constituents and mechanical properties.

Main Methods:

  • An arthroscopy-compatible Raman probe was used to acquire spectra from TEC constructs.
  • Multivariate linear decomposition extracted regression coefficient biomarkers for ECM constituents (sGAG, collagen, water) and scaffold material.
  • Raman acquisitions were performed during in vitro cultivation and analyzed for construct variability.

Main Results:

  • Raman spectroscopy did not affect chondrocyte growth in seeded-agarose constructs.
  • Raman-derived ECM biomarkers accurately reflected sGAG, collagen, and water content, explaining up to 90% of variation.
  • Biomarkers correlated strongly with construct stiffness (up to 94% variation explained).
  • Donor chondrocyte variability influenced ECM composition and stiffness, with biomarkers accounting for significant variation.

Conclusions:

  • The Raman spectroscopy platform enables non-destructive monitoring of TEC composition and mechanical properties.
  • This technology can optimize construct fabrication and improve preclinical evaluation for cartilage regenerative therapies.