Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jan 16, 2026

Preparation of DNA-crosslinked Polyacrylamide Hydrogels
09:06

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

15.2K

In Situ Stiffness Tunable DNA Hydrogels Based on Ring-Opening Polymerization.

Ziwei Shi1,2, Jiarui Li3, Miaomiao Qiu1,2

  • 1CAS Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.

Small Methods
|October 6, 2025
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The association of remnant cholesterol inflammatory index with the risk of major adverse cardiovascular events in patients with angina undergoing percutaneous coronary intervention: a retrospective study.

Frontiers in cardiovascular medicine·2026
Same author

Maxillary sinus-related adverse event reports associated with endosseous dental implants in the FDA MAUDE database: a retrospective text-mining analysis.

BMC oral health·2026
Same author

Synergistic modulation of composite donors and π-spacers in porphyrin sensitizers for enhanced charge transfer and photovoltaic efficiency: a DFT/TD-DFT study.

Physical chemistry chemical physics : PCCP·2026
Same author

Epidemiological characteristics of varicella in Xuzhou from 2015 to 2024: surveillance study.

BMC infectious diseases·2026
Same author

2-guanidine-4-methylquinazoline inhibits platelet activation and thrombosis by targeting the acid-sensing ion channel 3.

European journal of pharmacology·2026
Same author

Correction: Ice-assisted cryogenic embrittlement grinding for surfactant-free fabrication of soft nanomaterials.

Materials horizons·2026
This summary is machine-generated.

Researchers developed dynamic DNA hydrogels with tunable stiffness for artificial extracellular matrices. This biomimetic platform mimics natural tissue stiffening, offering programmable mechanical regulation under physiological conditions.

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Tissue Engineering

Background:

  • DNA hydrogels offer programmable and biocompatible artificial extracellular matrices (ECMs).
  • Current stiffness modulation methods are largely static, limiting dynamic regulation.
  • Responsive building blocks are needed for dynamic control of hydrogel properties.

Purpose of the Study:

  • To present a novel ring-opening polymerization strategy for in situ regulation of DNA hydrogel stiffness.
  • To develop a dynamic and programmable biomimetic platform for mimicking ECM stiffening.

Main Methods:

  • Utilized supramolecular dimer rings with functional domains for polymerization.
  • Employed complementary regions, flexible spacers, and sticky ends in ring design.
Keywords:
3D cell cultureDNA nanotechnologyDNA supramolecular hydrogelring‐opening polymerizationstiffness regulation

More Related Videos

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

7.2K
Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels
07:17

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels

Published on: June 30, 2023

2.3K

Related Experiment Videos

Last Updated: Jan 16, 2026

Preparation of DNA-crosslinked Polyacrylamide Hydrogels
09:06

Preparation of DNA-crosslinked Polyacrylamide Hydrogels

Published on: August 27, 2014

15.2K
DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

7.2K
Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels
07:17

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels

Published on: June 30, 2023

2.3K
  • Induced polymerization via linkers, network remodeling via trigger strands, and reversible stiffness reduction via strand displacement.

    • Main Results:

    • Achieved in situ regulation of DNA hydrogel stiffness through a ring-opening polymerization strategy.
    • Demonstrated dynamic stiffness enhancement via hybridization with trigger strands.
    • Enabled reversible stiffness reduction using strand displacement mechanisms.

    Conclusions:

    • The developed approach allows for dynamic and programmable mechanical regulation of DNA hydrogels.
    • This method provides a biomimetic platform capable of mimicking the dynamic stiffening observed in native ECM.
    • The strategy offers a new avenue for creating advanced biomaterials with tunable mechanical properties for tissue engineering applications.