Monitoring Chemotherapeutic Drugs Release from the Single Core-Shell Nanocarrier at a Monolayer Graphene-Based Total...

Area of Science:

• Nanotechnology
• Biomedical Engineering
• Materials Science

Background:

• Core-shell nanostructures are vital nanocarriers for biomedical uses like drug delivery and thermal therapy.
• Monitoring drug release at the single nanoparticle level is crucial due to heterogeneity and for nanomedicine design.

Purpose of the Study:

• To develop a sensitive platform for real-time, single-nanoparticle monitoring of chemotherapeutic drug release.
• To investigate the loading and release kinetics of doxorubicin from silica core-gold shell nanoparticles.

Main Methods:

• A monolayer graphene-based total internal reflection imaging platform was created.
• Real-time surface charge detection of oscillating nanoparticles under an electric field using Fourier transform filtering.
• Evanescent field utilized for high sensitivity and low noise detection.

Main Results:

• The platform successfully monitored doxorubicin loading and release from individual SiO2@Au CS nanoparticles.
• Kinetic constants for drug release were extracted at the single nanoparticle level.
• Thermal-induced drug release rates were examined, revealing heterogeneity.

Conclusions:

• The developed platform offers a sensitive tool for studying nanoparticle surface charge heterogeneity.
• This technology aids in understanding structure-activity relationships for nanomedicines.
• Provides guidance for investigating in vivo nanocarrier mechanisms.