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Wastewater inocula enhance biogas production from molasses stillage, with 50°C being the optimal temperature. Diverse microbial communities in wastewater inocula improve process stability and methane yields compared to agricultural systems.

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Area of Science:

  • Biotechnology and Bioengineering
  • Environmental Science
  • Microbiology

Background:

  • Molasses stillage is a nutrient-rich byproduct suitable for biogas production.
  • Understanding microbial community dynamics is crucial for optimizing anaerobic digestion.

Purpose of the Study:

  • To evaluate the biochemical methane potential (BMP) of molasses stillage.
  • To compare the efficacy of wastewater treatment plant (WWTP) and agricultural biogas plant (ABP) inocula.
  • To determine the optimal temperature and substrate load for stillage digestion.

Main Methods:

  • Biochemical methane potential (BMP) assays were conducted.
  • Experiments varied temperature (40, 50, 60 °C) and initial substrate load (ISL: 2, 5, 10 g·L-1).
  • Microbial community structure was analyzed using 16S rRNA gene sequencing.

Main Results:

  • WWTP inocula yielded higher methane (0.262-0.477 N m3·kgvs-1) than ABP inocula (0.192-0.378 N m3·kgvs-1).
  • Optimal performance was observed at 50 °C with WWTP inocula.
  • Inhibition occurred at 60 °C and high ISL (10 g·L-1), linked to reduced diversity and specific microbial shifts.

Conclusions:

  • Wastewater inocula provide greater process stability due to higher microbial diversity.
  • 50 °C is the thermal optimum for molasses stillage codigestion, balancing methane yield and microbial resilience.
  • Microbial community composition significantly influences biogas production efficiency.