Compact scattering-based light sheet microscopy probe using a custom miniature objective lens

We have developed a compact scattering-based light sheet microscopy (sLSM) probe capable of imaging unstained tissues with cellular resolution. In the compact sLSM probe, a custom miniature objective lens was developed to achieve a high lateral resolution, large field of view (FOV), and small field curvature. The measured resolution of the custom objective was 1.65-1.97 μm across the FOV of ± 1.0 mm. The compact probe had dimensions of 4 cm in width and height, and 10 cm in length. The compact sLSM probe achieved an axial resolution better than 5.6 μm over a depth range of 206.2 μm, and a lateral resolution of 1.9 μm. Preliminary results showed that the compact sLSM probe could visualize cellular details of fixed human anal epithelial tissues in a similar manner to a bench LSM device using off-the-shelf objective lenses.