Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

20.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
20.0K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

14.8K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
14.8K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>APOE</i> ε4-specific associations between agitation, cerebrospinal fluid biomarkers, and cognitive decline in cognitively impaired patients.

Journal of Alzheimer's disease : JAD·2026
Same author

Crafting the Future of Digitization: How and When Digital Leadership Promotes Public Employees' Proactive Service Performance.

Behavioral sciences (Basel, Switzerland)·2026
Same author

A Novel Model for Predicting Post-Craniotomy Meningitis Using Early Postoperative Risk Stratification: A Multi-Center Retrospective Study.

Infection and drug resistance·2026
Same author

Diagnostic utility of novel marker TRIM63 ISH in the workup of uterine perivascular epithelioid cell tumors.

Virchows Archiv : an international journal of pathology·2026
Same author

A single-subject finite element study on the influence of root dislocation direction on inferior alveolar nerve stress during mesially impacted mandibular third molar extraction.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft·2026
Same author

Histological study to assess CO<sub>2</sub> laser therapy for the treatment of vaginal atrophy.

European journal of obstetrics, gynecology, and reproductive biology·2026
Same journal

Characterizing close-focus lenses for microendoscopy.

Journal of optical microsystems·2023
See all related articles

Related Experiment Video

Updated: Jan 16, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.1K

Compact scattering-based light sheet microscopy probe using a custom miniature objective lens.

Jingwei Zhao1, Yongjun Kim2, Momoka Sugimura1

  • 1University of Arizona, James C. Wyant College of Optical Sciences, Tucson, Arizona, USA, 85721.

Journal of Optical Microsystems
|October 6, 2025
PubMed
Summary
This summary is machine-generated.

A new compact scattering-based light sheet microscopy (sLSM) probe images unstained tissues at cellular resolution. This innovative microscopy tool offers high resolution and a large field of view for biological imaging.

Keywords:
compact microscopecustom objective lensintrinsic scatteringlight sheet microscopyminiature objective lensunstained tissue imaging

More Related Videos

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.2K
Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
10:55

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

Published on: November 20, 2018

18.1K

Related Experiment Videos

Last Updated: Jan 16, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.1K
Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.2K
Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
10:55

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

Published on: November 20, 2018

18.1K

Area of Science:

  • Biomedical Engineering
  • Optical Microscopy
  • Cellular Imaging

Background:

  • Light sheet microscopy (LSM) enables high-resolution imaging of biological samples.
  • Existing LSM systems can be bulky and require specialized setups.
  • Imaging unstained tissues presents challenges in achieving cellular resolution.

Purpose of the Study:

  • To develop a compact scattering-based light sheet microscopy (sLSM) probe.
  • To achieve cellular resolution imaging of unstained tissues.
  • To create a portable and high-performance microscopy solution.

Main Methods:

  • Development of a custom miniature objective lens for high lateral resolution and large field of view (FOV).
  • Integration of components into a compact probe (4cm x 4cm x 10cm).
  • Characterization of the probe's axial and lateral resolution and imaging depth.

Main Results:

  • The custom objective achieved a lateral resolution of 1.65-1.97 μm across a ± 1.0 mm FOV.
  • The compact sLSM probe demonstrated an axial resolution < 5.6 μm over 206.2 μm depth.
  • Cellular details in fixed human anal epithelial tissues were visualized, comparable to benchtop LSM.

Conclusions:

  • A compact sLSM probe capable of high-resolution imaging of unstained tissues has been successfully developed.
  • The probe offers significant advantages in portability and performance for biological research.
  • This technology has potential applications in various fields requiring detailed tissue visualization.