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Updated: Jan 16, 2026

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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Competitive Sequestration of miR-1183 by lncRNA DDX11-AS1 Drives Gliomagenesis through E2F7 Activation

Jianwei Wang1,2, Xinzhi Yang3, Lvbiao Lin4

  • 1Department of Neurosurgery, Shenzhen Hospital, Southern Medical University, Shenzhen, 518100, China.

Oncology Research
|October 6, 2025
PubMed
Abstract

Insights

Long noncoding RNA DDX11-AS1 promotes glioma progression by activating the miR-1183/E2F7 pathway. This finding highlights DDX11-AS1 as a potential therapeutic target for treating this lethal brain malignancy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Glioma is a lethal primary brain malignancy with a poor prognosis.
  • The role of long noncoding RNA (lncRNA) DDX11-AS1 in glioma pathogenesis requires further investigation.
  • DDX11-AS1 has known oncogenic functions in other cancer types.

Purpose of the Study:

  • To characterize the oncogenic role of DDX11-AS1 in glioma.
  • To elucidate the molecular mechanism by which DDX11-AS1 contributes to glioma progression.

Main Methods:

  • Analyzed DDX11-AS1 expression in clinical glioma specimens and public datasets.
  • Investigated the functional impact of DDX11-AS1 on glioma cell proliferation and migration using in vitro knockdown and overexpression.
  • Assessed in vivo tumor growth in orthotopic glioma mouse models.
  • Performed competitive endogenous RNA (ceRNA) analysis to identify regulatory axes involving DDX11-AS1, miR-1183, and E2F7.
  • Conducted functional rescue experiments via miR-1183 inhibition.

Main Results:

  • DDX11-AS1 expression was significantly upregulated in clinical glioma specimens.
  • DDX11-AS1 knockdown suppressed glioma cell proliferation and migration in vitro, while overexpression enhanced these phenotypes.
  • In vivo studies confirmed DDX11-AS1 drives glioma tumor growth.
  • DDX11-AS1 acts as a ceRNA by interacting with miR-1183.
  • miR-1183 inhibition rescued the effects of DDX11-AS1 knockdown on glioma phenotypes and restored E2F7 expression.

Conclusions:

  • lncRNA DDX11-AS1 promotes glioma progression through the miR-1183/E2F7 axis.
  • DDX11-AS1 represents a potential therapeutic target for glioma treatment.
Keywords:
DDX11 antisense RNA 1E2F transcription factor 7Gliomacompetitive endogenous RNAmiR-1183

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