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Related Experiment Video

Updated: Jan 15, 2026

Sample Preparation for Mass Cytometry Analysis
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DLD-based on-chip sample handling for label-free single-cell MS cytometry.

Junwen Zhu1, Yingqi Yang1, Chenxi Cao1

  • 1State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing 100084, P. R. China. wwh@tsinghua.edu.cn.

Analytical Methods : Advancing Methods and Applications
|October 6, 2025
PubMed
Summary
This summary is machine-generated.

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This study introduces a novel microfluidic chip for label-free mass cytometry. It efficiently sorts and prepares single cells for analysis using deterministic lateral displacement, enabling sample-in-result-out mass spectrometry.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Microfluidics

Background:

  • Single-cell mass spectrometry (MS) is vital for biochemical analysis.
  • Label-free mass cytometry combines MS with microfluidics.
  • Efficient on-chip cell sample handling is critical but underdeveloped for MS cytometry.

Purpose of the Study:

  • To develop a passive microfluidic method for cell sample handling (sorting and desalting) for single-cell MS analysis.
  • To integrate deterministic lateral displacement (DLD) for hydrodynamic cell manipulation.
  • To create a sample-in-result-out system for consecutive MS cytometry.

Main Methods:

  • Utilized two sequential DLD arrays to focus cells by size and sort target cells.
  • Replaced cell solution with MS extraction solution on-chip.

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Related Experiment Videos

Last Updated: Jan 15, 2026

Sample Preparation for Mass Cytometry Analysis
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Published on: April 29, 2017

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A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

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  • Integrated an electrospray ionization capillary with an on-chip liquid metal alloy electrode for MS analysis.

    • Main Results:

    • Successfully demonstrated a passive cell sample handling system using DLD.
    • Achieved consecutive MS cytometry analysis with a sample-in-result-out workflow.
    • Collected satisfactory MS cytometry data to discriminate between three cell types.

    Conclusions:

    • The proposed DLD-based microfluidic chip offers an efficient method for single-cell sample handling in MS analysis.
    • This technology can serve as a stand-alone module or an integrated component for on-chip MS cytometry.
    • It establishes a new paradigm for efficient sample preparation in single-cell MS.