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Method to Study Induced Systemic Resistance Against Bacterial Pathogens in Orchids Primed by Root Colonization of
Galih Chersy Pujasatria1, Chihiro Miura2, Hironori Kaminaka3
1The United Graduate School of Agricultural Science, Tottori University, Tottori, Japan.
None:
Plant roots are not free of microbe colonization, including by mycorrhizal fungi. Upon mycorrhizal colonization of the root system, the aboveground parts, i.e., leaves, will display increased resistance against pathogens. This beneficial microbe-induced phenomenon is called induced systemic resistance (ISR), which is distinctive from systemic acquired resistance (SAR) and occurs after a nearby leaf is infected. Recently, it was found that root colonization of orchid mycorrhizal fungi primes the plant against a necrotrophic bacterial pathogen, as is widely known in arbuscular mycorrhiza. Here, we describe a detailed method to study ISR against a bacterial pathogen (Dickeya fangzhongdai) in an orchid (Bletilla striata) primed by root colonization of mycorrhizal fungi.
