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Preparation and Evaluation of Mouse Premature Ovarian Insufficiency Model

  • 0The First Hospital of Anhui University of Science and Technology'; 3379439263@qq.com.
Journal of Visualized Experiments : Jove +

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October 6, 2025

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October 6, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study establishes a reliable cyclophosphamide-induced mouse model for premature ovarian insufficiency (POI). The model mimics chemotherapy

Area Of Science

  • Reproductive Biology
  • Toxicology
  • Animal Models

Background

  • Premature ovarian insufficiency (POI) is a significant cause of female infertility.
  • Reliable animal models are crucial for understanding POI mechanisms and developing treatments.
  • Chemotherapy-induced ovarian damage is a major concern, necessitating effective models.

Purpose Of The Study

  • To present a standardized protocol for establishing and evaluating a cyclophosphamide (CTX)-induced POI mouse model.
  • To provide a reproducible experimental platform for studying chemotherapy's reproductive toxicity.
  • To facilitate the screening of ovarian-protecting drugs and fertility preservation strategies.

Main Methods

  • Six-to-eight-week-old female mice received intraperitoneal injections of cyclophosphamide (CTX).
  • Estrous cycles were monitored using vaginal smear cytology.
  • Serum hormone levels (estradiol, FSH, AMH) were quantified via ELISA.
  • Ovarian histopathology (follicular atresia) and granulosa cell apoptosis (cleaved caspase-3) were assessed.

Main Results

  • CTX treatment led to disrupted estrous cyclicity and significantly reduced estradiol and AMH levels.
  • Elevated follicle-stimulating hormone (FSH) concentrations were observed in treated mice.
  • Histological analysis revealed increased follicular atresia and enhanced granulosa cell apoptosis.

Conclusions

  • The CTX-induced mouse model effectively replicates key features of POI, including follicle depletion and hormonal imbalances.
  • This model accurately mimics chemotherapy-induced ovarian damage, offering a valuable tool for research.
  • The protocol is simple, cost-effective, and suitable for widespread adoption in experimental settings.