Insect cell expression system: advances in applications, engineering strategies, and bioprocess development

Area of Science:

• Biotechnology
• Molecular Biology
• Bioprocess Engineering

Background:

• Insect cell expression systems are increasingly recognized for biopharmaceutical production.
• These platforms are versatile for various applications, including vaccines and therapeutic proteins.

Purpose of the Study:

• To provide a comprehensive review of recent advancements in insect cell expression systems.
• To highlight emerging strategies and address technical challenges in the field.

Main Methods:

• Review of recent literature on insect cell expression systems.
• Focus on applications in vaccines, therapeutic proteins, and complex protein production.
• Emphasis on emerging strategies like larval systems and gene therapy vectors.

Main Results:

• Insect cell systems show promise for producing human and veterinary vaccines, therapeutic proteins, and complex proteins.
• Emerging strategies include larval expression, baculovirus-mediated antigen display, and gene therapy vectors.
• Innovations address bottlenecks in scalability, productivity, and regulatory compliance through improved infection dynamics, genome engineering, and bioprocess optimization.

Conclusions:

• Insect cell expression platforms offer significant potential for next-generation biomanufacturing.
• Continued innovation is crucial to overcome technical limitations and fully realize their capabilities.
• Further research in genome engineering and bioprocess optimization will enhance scalability and productivity.