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Related Experiment Video

Updated: Jan 15, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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13-Plex DeAla Isobaric Reagents for High-Throughput Proteome Quantification.

Peng-Kai Liu1, Ting-Jia Gu2, Shuling Xu2

  • 1Biophysics Graduate Program, University of Wisconsin-Madison, Madison, Wisconsin 53705, United States.

Analytical Chemistry
|October 7, 2025
PubMed
Summary
This summary is machine-generated.

We developed a new, affordable isobaric labeling reagent called DeAla for mass spectrometry. This cost-effective tool improves peptide fragmentation and protein identification, making large-scale quantitative proteomics more accessible.

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Isobaric labeling is crucial for quantitative proteomics.
  • Commercial isobaric tags are expensive, hindering large-scale studies.

Purpose of the Study:

  • Introduce a novel, cost-effective isobaric reagent (DeAla).
  • Evaluate DeAla's performance in mass spectrometry-based quantitative proteomics.

Main Methods:

  • Synthesized DeAla using diethylated alanine and β-alanine.
  • Optimized labeling efficiency and collision energy.
  • Performed comparative proteomic analyses using DeAla, DiLeu tags, and label-free methods.

Main Results:

  • DeAla tags enhance peptide fragmentation and protein identification.
  • Achieved 13-plex multiplexing with high resolution.
  • DeAla showed comparable performance to label-free methods and outperformed DiLeu tags.
  • Demonstrated accurate and reproducible quantification.

Conclusions:

  • DeAla is a cost-effective, high-performance isobaric tagging tool.
  • Enhances peptide fragmentation and protein identification.
  • Suitable for complex quantitative proteomic analyses.