Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activity-dependent lipid droplet biogenesis and turnover regulate synaptic integrity.

bioRxiv : the preprint server for biology·2026
Same author

Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion.

Science (New York, N.Y.)·2026
Same author

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same author

Targeted degradation of MDM2 overcomes feedback regulation of p53 signaling in Merkel cell carcinoma models.

The Journal of clinical investigation·2026
Same author

A human lysosomal storage disorder toolkit for decoding proteome landscapes in cortical-like and dopaminergic-like induced neurons.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Structural basis for regulation of the proteasome 20S core particle by the Parkinsonism-associated proteins FBXO7 and PI31.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jan 15, 2026

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
12:34

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

14.0K

Comparative Proteomic Profiling of Human Cell Lines Using Capillary and Micro-Pillar Array Columns.

Christina B Schroeter1,2, Joao A Paulo1

  • 1Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.

Proteomics
|October 7, 2025
PubMed
Summary
This summary is machine-generated.

Microfluidic pillar array columns (µPAC) offer comparable proteomic profiling performance to traditional capillary columns. These robust µPAC columns provide ease of use and durability for quantitative proteomics workflows.

Keywords:
AccucoreAscendTMTchromatographyµPAC

More Related Videos

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
08:08

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

16.9K
Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
09:02

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Published on: April 20, 2018

20.4K

Related Experiment Videos

Last Updated: Jan 15, 2026

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
12:34

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

14.0K
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
08:08

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

16.9K
Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
09:02

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Published on: April 20, 2018

20.4K

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Chromatographic column choice significantly influences proteomic profiling outcomes.
  • Limited comparative studies exist for novel column technologies versus conventional ones.

Purpose of the Study:

  • To compare the performance of microfabricated pillar array columns (µPAC) against conventional flame-pulled capillary columns.
  • To evaluate their utility in multiplexed quantitative proteomic experiments using TMTpro18-plex labeling.

Main Methods:

  • Global proteome profiling of six human cell lines in triplicate.
  • Utilized TMTpro18-plex for sample multiplexing.
  • Compared peptide and protein quantification, analytical parameters, and reproducibility between µPAC and Accucore columns.

Main Results:

  • Both µPAC and Accucore columns demonstrated comparable peptide and protein quantification yields and overlap.
  • Reproducible cell line-specific patterns were observed with both column types via clustering analyses.
  • Analytical metrics including XCorr scores, signal-to-noise ratio, and peak resolution were consistent across formats.

Conclusions:

  • Microfluidic µPAC columns are a viable alternative to traditional capillary columns for TMT-based quantitative proteomics.
  • µPAC columns offer advantages in ease of use and durability without compromising proteomic depth or quantitative accuracy.
  • This study provides evidence-based guidance for selecting columns to optimize proteomic experimental design and advance quantitative analysis.