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Updated: Jul 6, 2026
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
Colorimetric Aptasensor Based on Bimetallic Single-Atom Nanozyme and Self-Selected Aptamers for Ultrasensitive
Zhichao Liu1,2, Lei Ji2, Yan Li2
1College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao Application Technology Innovation Center of Photoelectric Biosensing for Clinical Diagnosis and Treatment, Qingdao University, Qingdao 266071, PR China.
Abstract:
Quarantine plant viruses (QPV) pose significant economic and environmental threats, yet their efficient and sensitive detection remains a major challenge. Herein, a sandwich colorimetric aptasensor strategy has been proposed based on a bimetallic single-atom nanozyme (FeCo-NC) and self-selected aptamers for the on-site detection of Tomato mosaic virus (ToMV), a prevalent QPV. The dual active centers of FeCo-NC significantly enhance the efficient activation of H2O2. Simultaneously, FeCo-NC serves as an excellent carrier for aptamers due to its large surface area and high porosity. Aptamers obtained through SELEX exhibit high efficiency and specificity in recognizing ToMV, ensuring high selectivity of the sensor. Moreover, a 96-well plate format enables high-throughput detection, enhancing detection efficiency. The sensor exhibits a low detection limit of 1.4 pg/mL and a wide linear range of 10-107 pg/mL. Compared with traditional detection methods, this sensing is highly versatile, making it valuable for plant disease monitoring and environmental protection.
