Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oxidation of Phenols to Quinones01:17

Oxidation of Phenols to Quinones

4.5K
In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
4.5K
  1. Home
  3. Research Domains
  5. Engineering
  7. Automotive Engineering
  9. Automotive Combustion And Fuel Engineering
  11. Vitamin K1 Attenuates Acetaminophen-induced Ferroptotic Hepatic Damage In Mice Via Targeting Keap1/nrf2/ho-1 Pathway.
  1. Home
  3. Research Domains
  5. Engineering
  7. Automotive Engineering
  9. Automotive Combustion And Fuel Engineering
  11. Vitamin K1 Attenuates Acetaminophen-induced Ferroptotic Hepatic Damage In Mice Via Targeting Keap1/nrf2/ho-1 Pathway.

Related Experiment Video

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

1.8K

Vitamin K1 attenuates acetaminophen-induced ferroptotic hepatic damage in mice via targeting keap1/Nrf2/HO-1 pathway.

Shimaa A Abass1,2, Ahmed A Mohamed3, Ahmed H Abd El-Slam4

  • 1Department of Biochemistry, Faculty of Pharmacy, Kaferelsheikh University, Kafr El-Sheikh, 33516, Egypt.

Naunyn-Schmiedeberg'S Archives of Pharmacology
|October 7, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Vitamin K1 (Vit K1) protects the liver from acetaminophen (APAP) injury by reducing ferroptosis and oxidative stress. It activates the Keap1-Nrf2/HO-1 pathway, offering potential as a therapeutic for drug-induced liver damage.

Keywords:
AcetaminophenFerroptosisHO-1Liver injury

More Related Videos

Partial Lobular Hepatectomy: A Surgical Model for Morphologic Liver Regeneration
05:37

Partial Lobular Hepatectomy: A Surgical Model for Morphologic Liver Regeneration

Published on: May 31, 2018

12.7K

Related Experiment Videos

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
04:01

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

1.8K
Partial Lobular Hepatectomy: A Surgical Model for Morphologic Liver Regeneration
05:37

Partial Lobular Hepatectomy: A Surgical Model for Morphologic Liver Regeneration

Published on: May 31, 2018

12.7K

Area of Science:

  • Hepatology
  • Toxicology
  • Cellular Biology

Background:

  • Acetaminophen (APAP) overdose causes acute liver injury via the toxic metabolite N-acetyl-p-benzoquinone imine (NAPQI).
  • NAPQI induces oxidative stress and ferroptosis, a cell death pathway driven by iron and lipid peroxidation.
  • The Kelch-like ECH-associated protein 1 (Keap1), nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) pathway regulates antioxidant responses.

Purpose of the Study:

  • To investigate the hepatoprotective effects of vitamin K1 (Vit K1) against APAP-induced liver injury.
  • To determine if Vit K1 modulates ferroptosis through the Keap1-Nrf2/HO-1 antioxidant pathway.

Main Methods:

  • Male mice were pretreated with varying doses of Vit K1 before APAP administration.
Nrf2
Vitamin K1
  • Liver damage was assessed using biomarkers for liver injury (ALT, AST, albumin), oxidative stress (GSH, MDA, NO), and ferroptosis (GPX4, hepatic iron, ACSL4).
  • Gene and protein expression of Keap1, Nrf2, and HO-1 were analyzed.

    • Main Results:

    • APAP significantly increased liver injury markers, oxidative stress, and ferroptosis indicators.
    • Vit K1 pretreatment dose-dependently ameliorated APAP-induced liver damage.
    • Vit K1 suppressed ACSL4 and Keap1 expression while upregulating Nrf2 and HO-1, indicating activation of the antioxidant pathway.

    Conclusions:

    • Vitamin K1 exhibits significant hepatoprotective effects against APAP-induced liver injury.
    • Vit K1 protects by inhibiting ferroptosis and activating the Keap1-Nrf2/HO-1 antioxidant pathway.
    • Vit K1 is a potential therapeutic candidate for managing drug-induced liver injury.