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lncRNA TMEM147-AS1 promotes acute myeloid leukemia development by regulating miR-873-3p/ZFX axis

Yong Xu1, Yanli Wang2

  • 1Department of Blood Transfusion, Zhoushan Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, Zhoushan, 316000, Zhejiang Province, China.

Journal of Molecular Histology
|October 7, 2025
PubMed

Insights

Long non-coding RNA TMEM147-AS1 is upregulated in acute myeloid leukemia (AML), promoting cancer progression. This lncRNA serves as a potential diagnostic and prognostic biomarker for AML patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Acute myeloid leukemia (AML) is a complex blood cancer characterized by abnormal myeloid cell proliferation.
  • Long non-coding RNAs (lncRNAs) play crucial roles in regulating AML pathogenesis.
  • TMEM147-AS1 is a lncRNA previously found to be dysregulated in AML.

Purpose of the Study:

  • To investigate the functional role of TMEM147-AS1 in AML progression.
  • To elucidate the molecular mechanism underlying TMEM147-AS1's action in AML.
  • To evaluate TMEM147-AS1 as a diagnostic and prognostic marker for AML.

Main Methods:

  • Quantitative real-time PCR to assess TMEM147-AS1 and miR-873-3p expression in AML tissues and cell lines.
  • Bioinformatic analysis and dual-luciferase reporter assays to confirm targeting relationships.
  • Cell proliferation and apoptosis assays to evaluate the functional impact of TMEM147-AS1.

Main Results:

  • TMEM147-AS1 expression was significantly upregulated in AML tissues and cell lines compared to healthy controls.
  • High TMEM147-AS1 levels correlated with adverse cytogenetics, shorter overall survival, and poorer prognosis in AML patients.
  • TMEM147-AS1 was found to target miR-873-3p, which in turn targets ZFX, promoting AML cell proliferation and inhibiting apoptosis.

Conclusions:

  • TMEM147-AS1 promotes AML progression by modulating the miR-873-3p/ZFX axis.
  • TMEM147-AS1 demonstrates potential as a valuable diagnostic and prognostic biomarker for acute myeloid leukemia.
Keywords:
Acute myeloid leukemiaApoptosisProliferationTMEM147-AS1miR-873-3p

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