Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phenotyping of post-fertilization sperm mitophagy determinants discovered in a mammalian gamete-based cell-free system.

Biological research·2026
Same author

Paternal dietary macronutrients affect the seminal vesicle fluid proteome and fetal development: a geometric framework for nutrition study in mice.

Proceedings. Biological sciences·2026
Same author

Ram sperm and seminal plasma alter immune transcriptome and cytokine secretion in ovine cervical explants ex vivo.

Reproduction (Cambridge, England)·2025
Same author

The koala (Phascolarctos cinereus) prostate: a proteomic perspective on gland segmentation.

Biology of reproduction·2025
Same author

Dynamic sperm proteome remodeling during epididymal maturation in a marsupial, Macropus giganteus†.

Biology of reproduction·2025
Same author

Towards a kingdom of reproductive life - the core sperm proteome.

Reproduction (Cambridge, England)·2025

Related Experiment Video

Updated: May 10, 2026

Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses
07:59

Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses

Published on: September 19, 2011

13.0K

Cold shock, chilling and cryopreservation impact sperm proteasome function.

Grace Voss1, Mikayla Crouch1, Michal Zigo2

  • 1School of Veterinary Science, The University of Queensland, Gatton, Australia.

Reproduction (Cambridge, England)
|October 7, 2025
PubMed
Summary

Sperm preservation alters proteasome function, impacting fertility. Chilling and freezing significantly change proteasome activities, with cryopreservation increasing some activities while oxidative stress decreases all. Further research is needed.

Keywords:
20Scryopreservationoxidative stressproteasomeramsperm

More Related Videos

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
07:06

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Published on: December 12, 2011

40.6K
Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
13:32

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Published on: March 4, 2018

8.7K

Related Experiment Videos

Last Updated: May 10, 2026

Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses
07:59

Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses

Published on: September 19, 2011

13.0K
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
07:06

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Published on: December 12, 2011

40.6K
Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
13:32

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Published on: March 4, 2018

8.7K

Area of Science:

  • Reproductive biology
  • Molecular biology
  • Biochemistry

Background:

  • Sperm preservation methods like cryopreservation can cause sublethal molecular damage, reducing fertility and assisted reproductive technology efficacy.
  • Oxidative stress is a key factor in cryopreservation-induced sperm damage, which somatic cells manage via proteasome activity.
  • The relationship between sperm proteasome function and cryopreservation-induced oxidative stress remains unexplored.

Purpose of the Study:

  • To investigate the impact of cold shock, chilled storage, and cryopreservation on ram sperm proteasome activity.
  • To explore the direct effects of oxidative stress on sperm proteasome function.
  • To describe novel molecular changes in sperm due to preservation.

Main Methods:

  • Ram spermatozoa were subjected to cold shock, chilled storage, or cryopreservation.
  • Fresh sperm were exposed to varying levels of hydrogen peroxide (H2O2) to simulate oxidative stress.
  • Sperm motility was assessed using computer-assisted sperm analysis.
  • Proteasome activities (caspase-like, chymotrypsin-like, trypsin-like) were measured using fluorogenic peptide substrates.
  • Proteasome subunit abundance was determined via western blotting.

Main Results:

  • Chilled storage significantly decreased caspase-like and chymotrypsin-like proteasome activities, while cryopreservation significantly increased them.
  • Trypsin-like activity decreased with cold shock and cryopreservation, and showed a trend of decrease with chilling.
  • All measured proteasome activities significantly decreased under induced oxidative stress.
  • Cryopreservation led to an apparent increase in proteasome subunit abundance, unlike chilled storage.

Conclusions:

  • Sperm proteasome function is demonstrably altered by oxidative stress and common preservation methods (chilling, freezing).
  • These alterations represent a new molecular change associated with sperm preservation.
  • The functional consequences of these observed changes in sperm proteasome activity on fertility require further investigation.