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Updated: Jan 15, 2026

Bimolecular Fluorescence Complementation BiFC Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
Published on: June 12, 2010
Droplet outbursts from onion cutting.
Zixuan Wu1, Alireza Hooshanginejad2,3, Weilun Wang1
1Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14853.
Cutting onions releases tear-inducing aerosols through a two-stage droplet process. Sharper blades and slower cutting reduce ejected droplets, minimizing kitchen contamination risks.
Area of Science:
- Biophysics
- Food Science
- Materials Science
Background:
- Onion cutting commonly causes eye irritation due to aerosolized irritants.
- The precise physical mechanisms driving this aerosol release are not well understood.
Purpose of the Study:
- To elucidate the mechanics of tear-inducing aerosol formation during onion cutting.
- To investigate the influence of cutting parameters on droplet ejection.
- To develop a model explaining onion rupture and droplet release.
Main Methods:
- High-speed imaging and characterization of droplet formation.
- Systematic variation of blade sharpness and cutting speed.
- Strain mapping of onion tissue during cutting.
- Development and validation of a bilayer fracture model using experimental and numerical methods, including Instron testing.
Main Results:
- Droplet formation follows a two-stage process: initial outburst and subsequent ligament fragmentation.
- Faster or blunter blades increase the number and energy of ejected droplets.
- The onion's epidermis acts as a fracture barrier, allowing significant mesophyll compression before rupture.
- The bilayer model accurately captures the rupture mechanism, with model predictions aligning with experimental fracture force data.
Conclusions:
- Blade sharpness and cutting speed critically influence aerosol release during onion preparation.
- Understanding these mechanics can inform practices to reduce airborne irritants and potential pathogen spread in kitchens.
- The study provides a foundational model for vegetable tissue rupture relevant to food preparation and processing.
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