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Thermoradiotherapy reverses proliferation, metastasis, and anoikis resistance in glioblastoma through ITGA5-PI3K/AKT

Ying Shi1, Hongda An2, Wenshuang Wang1

  • 1Department of Magnetic Resonance, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.

Translational Oncology
|October 7, 2025
PubMed
Abstract

Insights

Thermoradiotherapy effectively treats glioblastoma (GBM) by overcoming anoikis resistance. Targeting ITGA5 inhibits GBM proliferation and metastasis, offering a novel therapeutic strategy.

Area of Science:

  • Neuro-oncology
  • Cancer Biology
  • Radiotherapy Research

Background:

  • Glioblastoma (GBM) is a highly aggressive brain cancer with poor prognosis.
  • Anoikis resistance contributes to GBM's resistance to standard therapies.
  • Novel treatment strategies are urgently needed for GBM.

Purpose of the Study:

  • To evaluate thermoradiotherapy as an integrative approach for GBM.
  • To sensitize GBM's resistance to anoikis.
  • To identify therapeutic targets for thermoradiotherapy.

Main Methods:

  • Therapeutic effects assessed via cell counting kit-8, colony formation, Transwell, and anoikis assays.
  • Proteomic analysis identified ITGA5 as a potential therapeutic target.
  • Molecular mechanisms explored using EdU, Western blotting, and qRT-PCR.

Main Results:

  • Thermoradiotherapy inhibited GBM proliferation and metastasis, reversing anoikis resistance.
  • ITGA5 was identified as a therapeutic target promoting GBM proliferation, metastasis, and anoikis resistance.
  • ITGA5 regulates GBM progression via the PI3K/AKT pathway.

Conclusions:

  • Thermoradiotherapy demonstrates potent anti-tumor efficacy with minimal side effects for GBM.
  • ITGA5 is a key therapeutic target for thermoradiotherapy in GBM.
  • Targeting ITGA5 offers a promising strategy for GBM treatment.
Keywords:
Anoikis resistanceGlioblastomaHyperthermiaITGA5RadiotherapyThermoradiotherapy

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